FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-191-6-1-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-191-6-1-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117690
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC5102843.35797433936613No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC92737.8791741014529695No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC51094.341065511088453No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCT48974.160931260090067No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC45063.828702523578894No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC29052.4683490525958027No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC21671.8412779335542528No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC18521.573625626646274No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT15161.2881298326110968No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC14821.259240377262299No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC13461.1436825558671084No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTG8580.7290339026255417No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT7930.6738040615175461No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC7070.6007307332823519No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG6990.5939332143767525No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT6830.5803381765655535No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC6530.5548474806695556No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGC5570.4732772538023621No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTG5530.46987849434956236No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGT4870.41379896337836686No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA4710.400203925567168No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT4690.3985045458407681No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC4270.362817571586371No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC3840.32628090746877386No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTT3840.32628090746877386No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC3840.32628090746877386No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTT3830.325431217605574No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC3390.28804486362477694No Hit
CCACGTCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA3350.2846461041719772No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC2930.24895912991758007No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTG2880.2447106806015804No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC2530.2149715353895828No Hit
CCACGTCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGG2490.2115727759367831No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT2340.1988274279887841No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC2320.1971280482623842No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC2320.1971280482623842No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTGT2310.19627835839918428No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG2290.19457897867278445No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC2270.19287959894638457No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCT2230.18948083949358482No Hit
CCACGTCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTG2220.1886311496303849No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCC2150.1826833205879854No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC2050.17418642195598605No Hit
CCACGTCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT2020.17163735236638628No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG1800.15294417537598778No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTT1740.14784603619678816No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1660.14104851729118872No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC1650.14019882742798878No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1640.13934913756478887No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC1550.13170192879598946No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC1530.1300025490695896No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC1510.12830316934318975No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCTA1450.12320503016399013No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTG1420.12065596057439035No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG1410.11980627071119042No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCC1380.11725720112159062No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1310.11130937207919109No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCC1280.10876030248959129No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1200.10196278358399186No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG157.223887E-6295.04
CCACGTT700.0295.01
CCACGTC115500.0295.01
GCCTAGA108.4553903E-4294.999975
CACGTTT108.4553903E-4294.999972
CCACGGC108.4553903E-4294.999971
CGTCTAG114050.0294.741364
CACGTCT115200.0294.359832
ACGTCTA114850.0294.229433
GTCTAGA114050.0294.09475
CTAGAAC114950.0290.380587
TCTAGAA115700.0288.625766
TAGAACA119050.0280.62798
AGAACAG120450.0277.121229
CACGTTC400.0258.1252
ACGCCTA202.2769356E-5221.249983
GTCTGGA202.2769356E-5221.249985
CTAGACC353.954483E-9210.714287
TTCTAGA500.0206.56
ACGTTCT500.0206.53
Produced by FastQC (version 0.11.5)