FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-190-6-1-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-190-6-1-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112519
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC5001444.44938188217101No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC85407.589829273278291No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC53054.714759285098516No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG45854.074867355735476No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC42223.7522551746816095No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC33012.9337267483713863No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC19641.7454829850958502No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC19421.7259307316986463No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT13251.1775788977861517No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC11971.0638203325660556No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC11130.9891662741403674No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT9180.8158622099378772No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC8150.7243221144873311No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG8060.716323465370293No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC7510.6674428318772829No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCC6890.6123410268487989No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA6630.5892338182884669No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT6410.5696815648912628No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC6210.5519067890756229No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT5520.4905838125116647No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTA5060.44970182813569265No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC4090.3634941654298385No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC3860.34305317324185247No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC3380.30039371128431647No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACC3160.2808414578871124No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC3030.26928785360694635No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCC2990.26573289844381837No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG2770.24618064504661435No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC2480.2204072201139363No Hit
CCACGTCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA2220.1973000115536043No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC2150.19107884001813027No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC2060.18308019090109226No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC1940.17241532541170823No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1910.16974910903936224No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTAC1860.16530541508545224No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC1830.16263919871310623No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC1830.16263919871310623No Hit
CCACGTCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT1730.15375181080528622No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCC1700.15108559443294023No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC1700.15108559443294023No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTG1680.1493081168513762No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1640.1457531616882482No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1640.1457531616882482No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC1640.1457531616882482No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCC1610.14308694531590221No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT1610.14308694531590221No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGT1600.1421982065251202No Hit
CCACGTCTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGG1590.1413094677343382No Hit
CCACGTCTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGTA1540.1368657737804282No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC1430.12708964708182618No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACC1410.12531216950026217No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCC1390.12353469191869818No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC1370.12175721433713418No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG1270.11286982642931416No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTCC1260.11198108763853216No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC1240.11020361005696815No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC1230.10931487126618615No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCC1220.10842613247540415No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAACACCGCCAGGTCC1220.10842613247540415No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1190.10575991610305814No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1190.10575991610305814No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCC1160.10309369973071215No Hit
CCACGTCAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1130.10042748335836614No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1130.10042748335836614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTT600.0295.01
CACGTCT111100.0294.601682
CCACGTC111650.0294.471561
ACGTCTA111250.0293.674163
CGTCTAG110800.0293.535644
GTCTAGA110750.0293.534975
CTAGAAC111000.0290.4827
TCTAGAA111300.0290.361636
TAGAACA114900.0280.750648
AGAACAG115100.0280.00659
CCACGTA251.8746869E-7236.01
CACGTTT202.2766715E-5221.249982
CCACGCT202.2766715E-5221.249981
ACGTTAG500.0206.53
CGTTAGA500.0206.54
AGTACGG150.0028462952196.66667295
TCTAAAC551.8189894E-12187.727266
CGTCTGA255.5432607E-5177.04
CACGTTA603.6379788E-12172.083342
ACGTCAG750.0157.333343
Produced by FastQC (version 0.11.5)