FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-19-4-4-1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-19-4-4-1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT206017.058628684995032No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT142911.833388539251407No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC10438.63696588274263No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT7586.276912885061278No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT5714.728386883073866No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT2772.2938058959920506No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT2682.2192779065915866No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCTG2582.13646902947996No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC2271.8797615104339187No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTG2261.871480622722756No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACCC1971.6313348790990394No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCCT1801.4905597880092747No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT1691.3994700231864856No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT1641.3580655846306724No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1451.2007287181185824No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTGT1391.1510433918516065No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACCC1150.9523020867837031No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGTA910.7535607817157999No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACCC900.7452798940046373No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTG870.7204372308711493No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT770.6376283537595231No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCCT740.612785690626035No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCCT660.546538588936734No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTGT650.5382577012255714No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT630.5216959258032461No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT620.5134150380920834No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTAC570.4720105995362702No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACCCT550.455448824113945No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTGT550.455448824113945No Hit
AGGAATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTGT520.4306061609804571No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTGT500.41404438555813183No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG500.41404438555813183No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTACC460.38092083471348126No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG450.37263994700231867No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG450.37263994700231867No Hit
AGGAATAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTGTA430.3560781715799934No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACCCT430.3560781715799934No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTGTAC400.33123550844650546No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTGTAC390.32295462073534287No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG360.29811195760185494No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATCC340.28155018217952965No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTGT330.273269294468367No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTGT330.273269294468367No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC320.26498840675720436No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGTA320.26498840675720436No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTGT310.2567075190460417No Hit
AGGAATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTGTACCC310.2567075190460417No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA300.2484266313348791No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTGT300.2484266313348791No Hit
AGGAATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCTG280.23186485591255385No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT270.22358396820139118No Hit
AGGAATAGAACAGGCTCCTCTTAGGGGTATCTAATCCCAGTGTACCCTGT260.21530308049022856No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG260.21530308049022856No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC250.20702219277906592No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCTG250.20702219277906592No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA230.19046041735674063No Hit
AGGAATAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAATCCC230.19046041735674063No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTGTA220.18217952964557801No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCAGT220.18217952964557801No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTACCC220.18217952964557801No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTACC210.17389864193441537No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCCT210.17389864193441537No Hit
AGGAATAGAACAGGCTCCTCTATGGGTATCTAATCCCAGTGTACCCTGTC210.17389864193441537No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTACCC210.17389864193441537No Hit
AGGAATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTACC210.17389864193441537No Hit
AGGAATAGAACAGGCTCCTCTAGTCCAGCGTCAGGGGTATCTAATCCCAG200.16561775422325273No Hit
AGGAATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTGT200.16561775422325273No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT200.16561775422325273No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTACC190.15733686651209008No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT180.14905597880092747No Hit
AGGAATAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTGTACCC170.14077509108976483No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT150.12421331566743955No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACATGGGGTATCTAATCCCAGTG150.12421331566743955No Hit
AGGAATAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG150.12421331566743955No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGGGTATCTAATCCCAGTGTACC140.11593242795627692No Hit
AGGAATAGAACAGGCTCCTCTAGTTGTCGGGGGTATCTAATCCCAGTGTA140.11593242795627692No Hit
AGGAATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGTA130.10765154024511428No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG130.10765154024511428No Hit
AGGAATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTACCC130.10765154024511428No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTACC130.10765154024511428No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT130.10765154024511428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCT108.3615584E-4295.09
CGCGCAC108.3615584E-4295.0295
GGAATAG11800.0291.252
GAACAGG11750.0289.978738
AACAGGC11850.0287.531659
GAATAGA12050.0285.20753
AGGAATA12200.0284.118871
AATAGAA12350.0278.279364
ATAGAAC12400.0274.778235
AGAACAG12350.0274.696357
TAGAACA12350.0273.5026
GAACGGC150.0028149525196.666668
AGAACGG150.0028149525196.666667
GGAAAAG150.0028149525196.666662
AGGAAAA200.006655757147.51
CTACAGG501.8189894E-1259.00000425-29
TGTTGCT501.8189894E-1259.00000460-64
TCAAGGT501.8189894E-1259.000004105-109
GGTCCTT2150.059.00000440-44
GGTTTAC501.8189894E-1259.000004110-114
Produced by FastQC (version 0.11.5)