FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-189-6-1-1-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-189-6-1-1-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95416
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC4088842.85235180682485No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC73667.7198792655319854No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC52075.457156032531231No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT43164.523350381487382No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC35363.7058774209776137No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC27512.883164249182527No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC16091.686299991615662No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC15781.653810681646684No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC12381.2974763142449903No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC10851.1371258489142282No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG8350.875115284648277No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC7170.7514462983147481No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACC6880.7210530728598977No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC6800.7126687348033873No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT6070.6361616500377295No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC5310.5565104385008803No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC5020.52611721304603No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC5020.52611721304603No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT4890.5124926637042005No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC4050.42445711411084097No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTAC3800.39825605768424577No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA3530.3699589167435231No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC3310.34690198708811937No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC3240.33956569128867276No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC3230.33851764903160897No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC3010.3154607193762053No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC2560.26829881780833403No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC2540.2662027332942064No Hit
AATCCTATTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG2520.26410664878007883No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT2410.25257818395237697No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC2390.25048209943824934No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC2310.2420977613817389No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC2230.2337134233252285No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC2200.23056929655403705No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC2130.22323300075459043No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC2100.22008887398339902No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC2040.2138006204410162No Hit
AATCCTATTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGT1960.20541628238450574No Hit
AATCCTATTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT1940.20332019787037814No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC1910.20017607109918673No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA1750.18340739498616584No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG1710.17921522595791062No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTAC1630.17083088790140016No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATACGAAGCACCGCCAGGTC1590.16663871887314496No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC1580.16559067661608115No Hit
AATCCTATTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTACC1470.1540622117883793No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTC1450.1519661272742517No Hit
AATCCTATTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG1400.14672591598893267No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAG1190.12471702859059278No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTA1180.12366898633352898No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1160.12157290181940136No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1150.12052485956233756No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTC1150.12052485956233756No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTC1150.12052485956233756No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTC1110.11633269053408232No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC1060.11109247924876331No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACC1050.11004443699169951No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1000.10480422570638048No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA990.10375618344931668No Hit
AATCCTATTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC990.10375618344931668No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCC980.10270814119225287No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTA970.10166009893518907No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT970.10166009893518907No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTAC970.10166009893518907No Hit
AATCCTATTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC960.10061205667812527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATT94200.0295.000033
TTAGACC108.452886E-4295.08
ATCCATT157.2196126E-6294.999972
CCTATTG157.2196126E-6294.999974
ATCCTAT94800.0294.377622
CCTATTA94250.0294.21754
AATCCTA95300.0293.29751
ATTAGAA93750.0293.1127
CTATTAG95450.0289.12785
TATTAGA95500.0288.8226
TTAGAAC95200.0287.87298
TAGAACA96800.0282.352789
CCTATAG457.6397555E-11196.666674
ATCCTCT150.00284562196.666662
AGAATTG150.00284562196.66666295
CCATTAG255.5406334E-5177.04
ATTAGAC800.0165.93757
TCCTATA655.456968E-12158.846163
AATCCTC200.006728083147.51
CTATTGA352.1170081E-4126.4285665
Produced by FastQC (version 0.11.5)