FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-188-6-4-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-188-6-4-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93042
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT4305646.275875411104664No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT79518.545603060983211No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT36633.936931708260785No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT31993.438232196212463No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG26302.8266804238945853No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT18151.9507319275166055No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT14591.5681090260312547No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT10941.1758130736656565No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT10571.1360460867135274No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC9891.0629608133961008No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT8080.8684250123600095No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCT7660.8232841082521872No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT5460.5868317534016896No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT5430.5836074031082737No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT5310.5707100019346102No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTCCT4310.46323165882074757No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCT4270.4589325250961931No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT3930.4223898884374798No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT3840.4127168375572322No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCAT3770.4051933535392618No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGTCTGAGGCACCGCCAAGTCCT3690.3965950860901528No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC3630.3901463855033211No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATTTGGGGCACCGCCAAGTCCT3460.37187506717396446No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT3440.36972550031168716No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG3330.3579028825691623No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT3190.34285591453322156No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2900.31168719503020137No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG2770.2977150104253993No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATTTGAAGCACCGCCAGGTCCT2760.2966402269942606No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT2760.2966402269942606No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT2650.28481760925173577No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT2490.26762107435351773No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT2400.25794802347327017No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTT2390.2568732400421315No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCTT2290.24612540573074526No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTCCT2280.24505062229960664No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2270.24397583886846802No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2220.23860192171277486No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTG2180.23430278798822038No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCTGGTCCT2160.2321532211259431No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCC1990.2138819027965865No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGTT1970.21173233593430926No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1950.20958276907203197No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT1950.20958276907203197No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAATC1930.20743320220975472No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCGTACCACCAA1930.20743320220975472No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT1910.20528363534747748No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGACGACGACGACGAGACGGGGTAT1880.20205928505406162No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAG1820.19561058446722984No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC1780.19131145074267533No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTTGTGGCC1730.18593753358698223No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC1730.18593753358698223No Hit
GAGTGGTAGAACAGGCTCCTCTAGTATCTCCCATGGGCTCCCTGCCTCTG1720.18486275015584358No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1700.18271318329356634No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCAGT1650.1773392661378732No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTTGT1620.17411491584445735No Hit
GAGTGGTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCCT1560.1676662152576256No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1560.1676662152576256No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1550.16659143182648697No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAAGAATAAAGAACCGCCAAGTCTTT1540.16551664839534835No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC1510.16229229810193246No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGTGG1510.16229229810193246No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT1400.15046968035940758No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGCAGCGTCTTTGGTTGTCACCGGT1290.1386470626168827No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT1290.1386470626168827No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT1250.1343479288923282No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATC1250.1343479288923282No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT1230.13219836203005095No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCTAA1210.1300487951677737No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG1170.1257496614432192No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTTG1150.12360009458094194No Hit
GAGTGGTAGAACAGGCTCCTCTAGAATGATATAAAGCACCGCCAAGTCCT1140.12252531114980332No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG1070.11500182713183293No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1050.1128522602695557No Hit
GAGTGGTAGAACAGGCTCCTCTAGATTATTGAGATTGTGTTTTCCAAAGA1040.11177747683841704No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG1000.10747834311386256No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAGGCACCGCCAAGTCCT990.10640355968272394No Hit
GAGTGGTAGAACAGGCTCCTCTCTTATACACATCTCCGAGGGGTATCTAA980.1053287762515853No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT960.10317920938930805No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT940.10102964252703081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA91250.0294.515054
GAGTGGT92200.0294.20011
GGTAGAA91050.0293.866035
AGTGGTA92100.0293.558622
GTGGTAG92250.0291.48243
GAACAGG91350.0290.47899
GTAGAAC93700.0283.508546
TAGAACA94600.0280.967227
AGAACAG95900.0276.85098
GTGGTAA500.0265.53
GACAGGC351.2732926E-11252.857139
TGGTAAA500.0206.54
GAAAGGC553.110472E-10160.90919
GGTAGAC553.110472E-10160.90915
TGGAGCA200.006727812147.5295
GGCAGAA200.006727812147.55
ATGGCTG200.006727812147.5295
AGTGGCA200.006727812147.52
TGGCAGA200.006727812147.54
GACTAGG200.006727812147.5295
Produced by FastQC (version 0.11.5)