FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-187-6-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-187-6-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90877
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC4557950.15460457541512No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC59176.510998382428998No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT47605.237848960683121No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC32253.5487527097065263No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC25922.852206829010641No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC20082.209579981733552No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCC13761.5141344894747846No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC13721.5097329357263114No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA11811.2995587442367156No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC10811.189519900524885No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC9331.0266624118313765No Hit
CGATTCCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG9261.0189596927715483No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC8190.90121812999989No Hit
CGATTCCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA6860.7548664678631557No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC6740.7416618066177361No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATAAAGCACCGCCAAGTCC6420.7064493766299503No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC6350.6987466575701222No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTT5670.6239202438460777No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATTTGGGGCACCGCCAAGTCC5620.6184183016604862No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTGGGTCTGAGGCACCGCCAAGTCC4610.5072790695115376No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC4190.4610627551525689No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC4170.4588619782783323No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGACATAAAGCAACGCCAAGTCC4120.4533600360927407No Hit
CGATTCCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT3630.3994410026739439No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC3610.3972402257997073No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGTATATGAAGCACCGCCAGGTCC3590.39503944892547066No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAATGGGGTATCTAATCCCAGTTGT3380.3719312917459863No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG3360.36973051487174974No Hit
CGATTCCTAGAACAGGCTCCTCTATCGTCGGGGTATCTAATCCCAGTTGT2740.301506431770415No Hit
CGATTCCTAGAACAGGCTCCTCTTATACACATCTGACGCTGCCGACGAGG2520.2772978861538123No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2510.276197497716694No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTT2370.2607920595970377No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC2250.2475873983516181No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTTG2170.23878429085467168No Hit
CGATTCCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC1990.21897729898654228No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGAGAGGGGGTATCTAATCCCAGTT1970.21677652211230564No Hit
CGATTCCTAGAACAGGCTCCTCTGGAAGACGGGGTATCTAATCCCAGTTG1800.1980699186812945No Hit
CGATTCCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTT1760.1936683649328213No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1410.1551547696336807No Hit
CGATTCCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTG1380.15185360432232578No Hit
CGATTCCTAGAACAGGCTCCTCTAGGAAGGGGTATCTAATCCCAGTTGTG1100.1210427280830133No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1100.1210427280830133No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT940.10343651308912045No Hit
CGATTCCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT940.10343651308912045No Hit
CGATTCCTAGAACAGGCTCCTCTATAGGGATATGAAGCACCGCCGGGTCC920.10123573621488384No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGA108.452224E-4295.06
TCCTAGG108.452224E-4295.05
TCCTAGA88700.0295.05
ATTCCTA89100.0295.03
GATTCCT89300.0295.02
TCCCAGA108.452224E-4295.05
TTCCTAG88900.0294.834084
CCTAGAA88100.0294.832586
CGATTCC89950.0294.672061
CTAGAAC88900.0291.349827
TAGAACA91900.0282.480968
AGAACAG91850.0282.474129
TTCCAGA450.0262.222234
CCTAGAC650.0249.615396
GATTCGT301.5697879E-9245.833312
ATTCCTG251.8727042E-7236.03
GATTCCA500.0236.02
CTAGACC202.2752336E-5221.257
TTCCTAA202.2752336E-5221.254
ATTCGTA353.947207E-9210.714283
Produced by FastQC (version 0.11.5)