FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-186-6-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-186-6-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87954
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC4620352.530868408486256No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC1106112.575891943515929No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC36894.194237897082566No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC33923.856561384360006No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC27643.1425517884348637No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC22192.5229097027991907No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC14771.6792868999704391No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC9031.0266730336312164No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC7800.8868272051299543No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC6440.7322009232098597No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT5970.6787638993110034No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC5340.6071355481274302No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT4610.5241376173909089No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG3890.44227664460968236No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG3490.3967983263978898No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC2510.2853764467789981No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC2260.2569524978966278No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC2090.23762421265661596No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA1950.22170680128248857No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC1720.19555676831070787No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCC1680.1910089364895286No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTAC1650.18759806262364417No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT1560.17736544102599086No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCATGTCC1500.17054369329422198No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTT1270.14439366032244128No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT1260.14325670236714647No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC1160.13188712281419832No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC1070.12165450121654502No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGACATAAAGCACCGCCAAGTAG1060.12051754326125019No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1060.12051754326125019No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC1050.11938058530595538No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1040.11824362735066056No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1000.11369579552948132No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACAGCCAGGTCC1000.11369579552948132No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTCC980.1114218796188917No Hit
CCACGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT970.11028492166359688No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCC950.10801100575300725No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGATCC930.10573708984241761No Hit
CCACGTTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC900.10232621597653319No Hit
CCACGTCTAGAACAGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT890.10118925802123838No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC880.10005230006594355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG206.152732E-8295.000033
ACGTCAG206.152732E-8295.000033
CCACGTT206.152732E-8295.000031
CGTCAGA206.152732E-8295.000034
ACGTTCT157.217697E-6295.03
CGTTCTA157.217697E-6295.04
CACGTCT86450.0294.829382
CGTCTAG85850.0294.82824
CCACGTC86900.0294.660521
ACGTCTA86250.0294.657963
GTCTAGA86050.0294.14295
CTAGAAC86350.0291.583657
TCTAGAA87400.0288.249426
TAGAACA88450.0283.993778
AGAACAG89400.0280.645979
CACGTTC202.274987E-5221.250022
CCACGCT202.274987E-5221.250021
CGTCTAA353.947207E-9210.714284
CAGCAGG150.0028452433196.66667295
CACGCCT150.0028452433196.666672
Produced by FastQC (version 0.11.5)