FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-185-6-1-2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-185-6-1-2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88607
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT3834543.27536199171623No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT68247.701423138126784No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT36284.0944846343968315No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT35854.0459557371313775No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTGT32543.672396086088007No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT27553.1092351620075163No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT19482.198471904025641No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT15511.7504260385748305No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT10801.2188653266671934No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT9791.1048788470436872No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT7900.8915774148769284No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT6880.776462356247249No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT5190.5857325042039568No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT5030.5676752401051836No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG4800.541717922963197No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT4790.5405893439570237No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGCT4660.5259178168767704No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT4480.5056033947656506No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCTG4300.48528897265453064No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTG4160.4694888665681041No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT4150.4683602875619307No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT3510.39613123116683785No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTGTT2890.32615933278409154No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCTT2850.3216450167593983No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTTG2710.30584491067297165No Hit
CTAGGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTGT2650.2990734366359317No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT2490.2810161725371585No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTTGC2370.26747322446307853No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT2150.24264448632726532No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTGC2070.23361585427787873No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT2050.23135869626553207No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT1950.22007290620379877No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT1940.21894432719762547No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTGC1770.19975848409267888No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT1750.19750132608033225No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1680.18960127303711896No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTTGC1640.18508695701242567No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT1630.18395837800625234No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT1610.18170121999390568No Hit
CTAGGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGTG1600.18057264098773235No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT1570.17718690396921236No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT1530.17267258794451906No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT1440.16251537688895912No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCCT1430.1613867978827858No Hit
CTAGGTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAGT1420.16025821887661246No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT1380.15574390285191914No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCTAGGTCCTT1290.1455866917963592No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT1280.14445811279018586No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG1240.13994379676549257No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT1210.1365580597469726No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1200.13542948074079925No Hit
CTAGGTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTGTTG1140.1286580067037593No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT1120.12640084869141266No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT1090.12301511167289265No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT1060.11962937465437268No Hit
CTAGGTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTGTG1030.1162436376358527No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTC1010.11398647962350605No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTGCT1010.11398647962350605No Hit
CTAGGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTTG1000.11285790061733272No Hit
CTAGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT970.10947216359881273No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCTAAT920.10382926856794611No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCTT900.10157211055559945No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA900.10157211055559945No Hit
CTAGGTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGTG890.10044353154942613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGC108.45187E-4295.09
GAACCGG108.45187E-4295.08
GGTAGGA108.45187E-4295.04
CTAGGTA87650.0294.495151
TAGGTAG87150.0294.3232
GGTAGAA86800.0293.81054
AGGTAGA87200.0293.30853
AACAGGC86900.0290.926369
GAACAGG86900.0290.756628
TAGAACA89450.0282.467866
GTAGAAC90150.0281.58355
AGAACAG90450.0279.5087
AGGTTAG450.0229.444443
GAACGGC202.2750435E-5221.258
CTAGGTT650.0204.230771
AGGTAGG150.0028452787196.666673
CGGAAGG150.0028452787196.66667295
AACGCGG150.0028452787196.66667295
TAGGGAG150.0028452787196.666672
TAGGTAA800.0184.3752
Produced by FastQC (version 0.11.5)