FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-184-6-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-184-6-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87214
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT5084058.293393262549586No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT63227.248836196023574No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT31883.655376430389616No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT30593.507464397917766No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT21562.4720801706148094No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT17171.9687206182493637No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC12921.4814135345242736No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT9131.0468502763317815No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT6780.7773981241543788No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG6680.7659320751255533No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCTAGGTCCT6180.7086018299814251No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG5900.6764968927007132No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT5590.6409521407113536No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC4150.4758410346962644No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT4050.4643749856674387No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTATGAGGCACCGCCAAGTCCT3620.4150709748434884No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT3520.4036049258146628No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC3480.39901850620313256No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTTCT3300.37837961795124636No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT3280.3760864081454812No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT3140.3600339395051253No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT3100.3554475198935951No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2800.3210493728071181No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT2530.29009104042928885No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG2530.29009104042928885No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC2210.2533996835370468No Hit
TGCTTATAGAACAGGCTCCTCTAGCTTAGGGGGGTATCTAATCCCAGTTG2010.23046758547939553No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT2010.23046758547939553No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT1890.21670832664480474No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT1840.2109753021303919No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGTATATGAAGCACCGCCAGGTCCT1790.20524227761597907No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTATGAGGCACCGCCAAGTACT1730.19836264819868368No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT1520.17428394523814983No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT1480.16969752562661958No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTG1370.15708487169491137No Hit
TGCTTATAGAACAGGCTCCTCTAGCCGAGGGGTATCTAATCCCAGTTGTG1290.14791203247185086No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA1250.14332561286032058No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT1140.13071295892861237No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1030.11810030499690416No Hit
TGCTTATGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1020.1169537000940216No Hit
TGCTTATAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1010.11580709519113903No Hit
TGCTTATAGAACAGGCTCCTCTGGGTGGGTCTGAGGCACCGCCAAGTCCT1000.11466049028825648No Hit
TGCTTAAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT980.11236728048249134No Hit
TGCTTATAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT970.11122067557960877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTT157.2174844E-6295.01
TGCTTAT86200.0295.01
GCTTAAG255.275069E-10295.02
GAACGGC157.2174844E-6295.09
GCTTTAG255.275069E-10295.02
CGAACGG108.451643E-4294.99997295
CATGAGG108.451643E-4294.99997295
AGAACGG206.152368E-8294.999978
GACCAGG108.451643E-4294.999979
CAAGCGG108.451643E-4294.99997295
CGAGCGC108.451643E-4294.99997295
CTTATAG85500.0294.482483
TTATAGA85500.0294.137454
GCTTATA86200.0293.97332
GAACAGG84950.0291.874669
TATAGAA86650.0288.70175
ATAGAAC86950.0286.178836
TAGAACA87600.0283.55027
AGAACAG87700.0283.058728
TGCTTAG202.2749215E-5221.249981
Produced by FastQC (version 0.11.5)