FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-183-6-2-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-183-6-2-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75721
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC3744849.45523698841801No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC777310.265316094610478No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC42475.608747903487804No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC37684.9761624912507765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT18862.4907225208330583No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC16012.1143408037400455No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC8451.1159387752406862No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC7641.0089671293300406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC7490.9891575652725135No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC7410.9785924644418326No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC6990.923125685080757No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC6860.9059573962309003No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG6150.812192126358606No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT6120.8082302135471006No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT5980.7897412870934086No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACGGCCAAGTCC5940.7844587366780682No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5260.6946553796172792No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT4430.5850424584989633No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG4300.5678741696491065No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC3940.5203312159110418No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTT3270.431848496454088No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGTACTGCCAAGTCT3200.4226040332272421No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC3200.4226040332272421No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT3000.3961912811505395No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCC2800.36977852907383685No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTATAAGAGACAGGACTACCGGGG2630.3473276898086396No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC2530.3341213137702883No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC1910.25224178233251016No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG1820.24035604389799395No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAATCACCGCCAGGTCC1820.24035604389799395No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCC1670.220546479840467No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC1300.1716828884985671No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1150.15187332444104015No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGG1060.13998758600652395No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1050.1386669484026888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT350.0295.000031
GCGAAGT74900.0295.01
CCTACGA108.449442E-4295.0295
CGAAGTT74550.0295.02
CGAAATT157.21373E-6294.999972
GTTAGAA73800.0294.600286
AAGTTAG74050.0294.402444
GAAGTTA74500.0294.208043
AGTTAGA74150.0294.00545
TTAGAAC76900.0282.724337
TAGAACA77000.0282.357158
AGAACAG78050.0278.558629
AAGTTGA251.8706669E-7236.04
GAAGTTG251.8706669E-7236.03
GTTAAAC403.45608E-11221.256
GAATTAG500.0206.53
GAAATTA150.0028444636196.666663
GCGAAGG150.0028444636196.666661
GTTAGAC304.6417153E-7196.666666
AGTTGAA304.6417153E-7196.666665
Produced by FastQC (version 0.11.5)