FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-182-6-5-8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-182-6-5-8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72237
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2651436.7041820673616No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT49566.8607500311474725No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT45766.334703822141008No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT34444.767639852153328No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT28843.9924138599332757No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCAC23573.2628708279690466No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT18492.559630106455141No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT13031.8037847640405886No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT12891.7844041142350873No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT11561.6002879410828248No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG11121.5393773274083917No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG9741.3483394936113071No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA8561.1849883023935102No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAAT7651.0590140786577515No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCGCCGCCAAGTCCC7631.056245414399823No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT7190.9953348007253899No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTG6630.9178122015033846No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT6150.8513642593130943No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAATC5610.7766103243490179No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT5050.6990877251270124No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT4740.6561734291291166No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCT4710.6520204327422235No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT4610.6381771114525797No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT4360.6035688082284701No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAAT3860.5343522017802511No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGTCACCGCCAAGTCCT3800.5260462090064648No Hit
GAGACTTAGAACAGGCTCCTCTTGCACTAAGGGCTCTTGGAGAACCGAAG3600.49835956642717716No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT3430.4748259202347827No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2930.4056093137865637No Hit
GAGACTTAGAACAGGCTCCTCTAGGACGGGGTATCTAATCCCAGTGTCAA2790.3862286639810623No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA2420.33500837520938026No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACT2410.3336240430804159No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT2170.3004000719852707No Hit
GAGACTTAGAACAGGCTCCTCTATCTCTCTGGGGTATCTAATCCCAGTGT1910.2644074366321968No Hit
GAGACTTAGAACAGGCTCCTCTAGAGTGATATAAAGCACCGCCAAGTCCT1580.2187244763763722No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT1520.21041848360258594No Hit
GAGACTTAGAACAGGCTCCTCTGGGGTATCTAATCCCAGTGTCAATCACT1400.19380649805501338No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAAGTCCT1360.18826916953915582No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGAACAGCCAAGTCCT1240.17165718399158328No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1080.14950786992815318No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT1050.14535487354126003No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT920.12735855586472308No Hit
GAGACTTAGAACAGGCTCCTCATAATCAGAGTCAATTCCGGTGAAGTTTT910.12597422373575867No Hit
GAGACTTAGAACAGGCTCCTTTAGAGGGATATGAAGCACCGCCAGGTCCT860.11905256309093677No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCA750.10382490967232859No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT740.10244057754336422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA70850.0295.000035
GAAAGGC157.2123494E-6295.09
CTTAGAC108.4486353E-4294.999975
ACTTAGA71050.0294.792424
GAGACTT71900.0294.17941
AGACTTA71400.0294.173682
GACTTAG71250.0294.17193
GAACAGG71000.0291.88389
TTAGAAC72300.0288.063636
TAGAACA73100.0284.507547
AGAACAG73250.0283.924938
GACTTGA351.2732926E-11252.857163
CTTGAAC301.5661499E-9245.833345
ACTTGAA403.45608E-11221.249984
AGACTTG500.0206.52
AGACTTT150.0028441919196.666672
AGAACGG150.0028441919196.666678
GAACGGC150.0028441919196.666679
TTGAACA408.752977E-9184.374986
GACTTAA255.5350793E-5177.03
Produced by FastQC (version 0.11.5)