FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-181-6-2-6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-181-6-2-6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72070
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT3581149.6891910642431No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT915512.702927709171638No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT49396.853059525461356No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT36965.1283474399889No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT32474.505342028583321No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT13461.8676286943249618No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT12661.7566255029832107No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG8481.1766338282225615No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCTAAGTCCTT6660.9241015679200777No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT6640.9213264881365338No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT5820.8075482170112391No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG4430.6146801720549466No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAACACCGCCAGGTCCTT4060.5633411960593867No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT3730.5175523796309144No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT3290.4565006243929513No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT3150.4370750659081449No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT2820.39128624947967255No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC2400.3330095740252532No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT2280.3163590953239906No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1650.2289440821423616No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTC1240.17205494657971415No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1210.16789232690439848No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT1150.15956708755376717No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT930.12904120993478563No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT780.1082281115582073No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG770.1068405716664354No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT750.10406549188289164No Hit
CTAGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT740.10267795199111975No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTC730.10129041209934785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAG108.4485975E-4295.0295
GAAAGGC157.2122875E-6295.08
GGTGAAC157.2122875E-6295.04
CTAGGTA71300.0294.586241
TAGGTAG70850.0294.583622
GGTAGAA70550.0294.581854
AGGTAGA70850.0294.375433
GAACAGG70250.0292.90048
AACAGGC70500.0291.234049
GTAGAAC71150.0290.231935
AGAACAG72950.0283.07067
TAGAACA73000.0282.87676
GGTAAAC400.0258.1254
TAGGTAA400.0258.1252
AGGTAAA450.0229.444463
GGAGAAC202.2732853E-5221.254
TAGGTTA202.2732853E-5221.252
CTAGCGA202.2732853E-5221.25295
ACGCATG150.0028441797196.66667295
AAAAGGC150.0028441797196.666679
Produced by FastQC (version 0.11.5)