FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-180-6-8-1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-180-6-8-1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71877
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT3410647.450505725058086No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT50236.988327281327823No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT31464.376921685657443No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT29704.132058934012271No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT28193.9219778232257885No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT17062.3734991721969476No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT11341.5776952293501396No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT11041.5359572603197127No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT10701.4886542287518956No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT8721.2131836331510775No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT6290.875106084004619No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG5060.7039804109798684No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT4750.6608511763150938No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT4440.6177219416503194No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG4110.5718101757168496No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT3720.5175508159772946No Hit
GAGACTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTAC3620.503638159633819No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT3210.4465962686255687No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT3200.4452050029912212No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA2740.3812067838112331No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT2690.37425045563949527No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT2410.3352950178777634No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT2000.2782531268695132No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT1970.2740793299664705No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCC1930.26851426742908024No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC1780.24764528291386675No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACCC1610.2239937671299581No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC1610.2239937671299581No Hit
GAGACTTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGG1580.21981997022691543No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCT1480.20590731388343977No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT1340.18642959500257383No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCT1320.1836470637338787No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACCC1300.18086453246518358No Hit
GAGACTTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTA1300.18086453246518358No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCT1270.17669073556214088No Hit
GAGACTTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG1250.17390820429344575No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCT1250.17390820429344575No Hit
GAGACTTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGT1200.16695187612170792No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCCT1150.1599955479499701No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1060.147474157240842No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT1030.1433003603377993No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCT1020.14190909470345173No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT1000.1391265634347566No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT1000.1391265634347566No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT980.13634403216606147No Hit
GAGACTTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTGT970.1349527665317139No Hit
GAGACTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT920.12799643835997607No Hit
GAGACTTAGAACGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT910.1266051727256285No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT890.12382264145693338No Hit
GAGACTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT890.12382264145693338No Hit
GAGACTTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTAT890.12382264145693338No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT870.12104011018823824No Hit
GAGACTTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTACC850.11825757891954311No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT840.11686631328519555No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT830.11547504765084798No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG810.11269251638215283No Hit
GAGACTTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGT790.10990998511345772No Hit
GAGACTTAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGGT780.10851871947911015No Hit
GAGACTTAGAACAGGCTCCTCTAGAGAGATATGAAGCACCGCCAGGTCCT770.10712745384476258No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT730.10156239130737232No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTT108.448549E-4295.02
GGTGAGG108.448549E-4295.0295
GAACGGC157.2122057E-6294.999979
GAGACTT71500.0294.793731
GACTTAG71200.0294.378543
CTTAGAA71000.0294.1695
AGACTTA71550.0293.969272
ACTTAGA71300.0293.965644
GAACAGG70950.0292.921059
TTAGAAC72150.0288.253666
TAGAACA73500.0282.357157
AGAACAG74200.0280.090978
TTAGAAA353.939931E-9210.714286
GGCAAGA150.0028441637196.66666295
GAAAGGC304.6400783E-7196.666669
CGAGGCA150.0028441637196.66666295
CGATCGA150.0028441637196.66666295
CTTAGAC304.6400783E-7196.666665
AGAACGG255.5349683E-5177.08
GACTTAA255.5349683E-5177.03
Produced by FastQC (version 0.11.5)