FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-18-5-4-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-18-5-4-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11784
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT353529.998302783435165No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT9017.645960624575696No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT8747.4168363883231505No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG7926.720977596741344No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC3452.9276985743380854No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2652.2488119484046165No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2652.2488119484046165No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG2592.197895451459606No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACCC2231.8923964697895452No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG1771.5020366598778006No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCCT1711.4511201629327901No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1511.2813985064494229No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC1481.2559402579769179No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTACC1341.1371350984385609No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1311.1116768499660556No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1160.9843856076035303No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1160.9843856076035303No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1080.9164969450101833No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA800.6788866259334692No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG700.5940257976917854No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT650.5515953835709436No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT520.44127630685675495No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA510.4327902240325866No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT510.4327902240325866No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT500.4243041412084182No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT480.40733197556008144No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT450.3818737270875764No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT430.3649015614392397No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT400.3394433129667346No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTG390.33095723014256617No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTACC340.28852681602172436No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTAC340.28852681602172436No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT330.280040733197556No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTACC320.2715546503733876No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTGTACC320.2715546503733876No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTA310.26306856754921926No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA280.23761031907671418No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT270.22912423625254583No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGTATCTAATCCCAGTGTACC270.22912423625254583No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACCC260.22063815342837748No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTAC260.22063815342837748No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT250.2121520706042091No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT240.20366598778004072No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT240.20366598778004072No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTACCC230.19517990495587237No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT230.19517990495587237No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT220.18669382213170402No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTG220.18669382213170402No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTGTA210.17820773930753564No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT210.17820773930753564No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACCC210.17820773930753564No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTGTAC210.17820773930753564No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTGTAC200.1697216564833673No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT200.1697216564833673No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT190.1612355736591989No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT190.1612355736591989No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTAC180.15274949083503053No Hit
TGCTTATAGAACAGGCTCCTCTAGTCCACGGGGGTATCTAATCCCAGTGT180.15274949083503053No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTG170.14426340801086218No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGTGGGGGTATCTAATCCCAGTG160.1357773251866938No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT160.1357773251866938No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTG160.1357773251866938No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA150.12729124236252545No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA150.12729124236252545No Hit
TGCTTATAGAACAGGCTCCTCTAGAACGAGAATGGGGTATCTAATCCCAG140.11880515953835709No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT130.11031907671418874No Hit
TGCTTATAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTGTACCC130.11031907671418874No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACCC120.10183299389002036No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT120.10183299389002036No Hit
TGCTTATAGAACAGGCTCCTCTTATACAGGGGTATCTAATCCCAGTGTAC120.10183299389002036No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTACCCT120.10183299389002036No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT120.10183299389002036No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTAC120.10183299389002036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTT108.358896E-4295.01
TGCTTAT11650.0295.01
GCTTTTA108.358896E-4295.02
GCTTATA11650.0295.02
CTTATAG11500.0294.999973
TTATAGA11550.0293.722964
GAACAGG11550.0292.44599
TATAGAA11650.0288.669535
ATAGAAC11700.0287.435886
TAGAACA11900.0283.844547
AGAACAG12500.0270.228
AGCGAGA200.0066536483147.5295
TATAGAC200.0066536483147.55
CGTTTGT257.742468E-659.00000460-64
ACTGTTG4500.059.000004100-104
GGATGTG150.00435987759.00000425-29
TCCTTTG6200.059.00000445-49
GTTTGTG257.742468E-659.00000460-64
GGGTTTT1200.059.00000450-54
GGGTTTA150.00435987759.000004110-114
Produced by FastQC (version 0.11.5)