FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-179-6-3-8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-179-6-3-8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68852
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT3076944.68860744785918No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT46196.708592342996572No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT36085.24022541102655No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT28704.168361122407483No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCAC21713.1531400685528377No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT16842.4458258293150523No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT15842.300586765816534No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT15302.222157671527334No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT9681.4059141346656596No Hit
TGCTTATAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAGGGGTA7471.0849358043339337No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAATC7361.0689595073490967No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAAT7141.0370069133794224No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT6040.8772439435310522No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTCA5560.8075291930517632No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT4900.7116714111427409No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT4880.7087666298727706No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT4080.5925753790739557No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTCAA3400.4938128158949631No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCAA3070.445883924940452No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCAAT2820.4095741590658224No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2810.4081217684308372No Hit
TGCTTATAGAACAGGCTCCTCTAGCCCACGAAACGGGGTATCTAATCCCA2550.37035961192122235No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGCCCT2520.36600244001626675No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTTCT2480.36019287747632606No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTTCCAAGTCCT2480.36019287747632606No Hit
TGCTTATAGAACAGGCTCCTCTACACATCTGACGCTGCGACGAGGGGTAT2460.35728809620635565No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT2360.3427641898565038No Hit
TGCTTATAGAACAGGCTCCTCTAGACGAGACGGGGGTATCTAATCCCAGT2180.31662115842677047No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG2070.3006448614419334No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG1990.2890257363620519No Hit
TGCTTATAGAACAGGCTCCTCTAATCCCACTAGACGGGGTATCTAATCCC1890.2745018300122001No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTGTCAAT1870.27159704874222973No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCT1810.2628827049323186No Hit
TGCTTATAGAACAGGCTCCTCTAACAGGGGTATCTAATCCCAGTGTCAAT1770.2570731423923779No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCAGT1680.24400162667751116No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGCCCT1680.24400162667751116No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1670.24254923604252598No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT1670.24254923604252598No Hit
TGCTTATAGAACAGGCTCCTCCTCAGGGGTATCTAATCCCAGTGTCAATC1660.2410968454075408No Hit
TGCTTATAGAACAGGCTCCTCTAGCCACGAACGGGGTATCTAATCCCAGT1620.2352872828676001No Hit
TGCTTATAGAACAGGCTCCTCTATCGTGGGGGTATCTAATCCCAGTGTCA1530.2222157671527334No Hit
TGCTTATAGAACAGGCTCCTCTAGACCACGAGACGGGGTATCTAATCCCA1500.21785859524777784No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCA1480.2149538139778075No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCT1400.20333468889792597No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGGGGTATCTAATCCCAGTGTCAA1390.2018822982629408No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCAA1370.19897751699297045No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCGAGGGGTATCTAATCCCAGTGTC1370.19897751699297045No Hit
TGCTTATAGAACAGGCTCCTCTATCTGTGGGGTATCTAATCCCAGTGTCA1350.19607273572300005No Hit
TGCTTATAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTC1250.1815488293731482No Hit
TGCTTATAGAACAGGCTCCTCGTCTCGTGGGGTATCTAATCCCAGTGTCA1250.1815488293731482No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATTTCGGGGTATCT1230.17864404810317783No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAAT1210.17573926683320745No Hit
TGCTTATAGAACAGGCTCCTCGAGACAGGGGTATCTAATCCCAGTGTCAA1090.15831057921338523No Hit
TGCTTATAGAACAGGCTCCTCTAGCCCACGGGGTATCTAATCCCAGTGTC1050.15250101667344448No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGGTATCTAATCCCAGTGTCA1020.14814384476848894No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGGTATCTAATCCCAGTGTCAA1010.14669145413350376No Hit
TGCTTATAGAACAGGCTCCTCTAGCCACAGGGGGTATCTAATCCCAGTGT980.1423342822285482No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGAGGGGGTATCTAATCCCAGTGTC880.12781037587869634No Hit
TGCTTATAGAACAGGCTCCTCTATACGGGGTATCTAATCCCAGTGTCAAT840.12200081333875558No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAATC770.11183407889385928No Hit
TGCTTATAGAACAGGCTCCTCTAGAGACCACGAGACGGGGTATCTAATCC750.10892929762388892No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT740.10747690698890375No Hit
TGCTTATAGAACAGGCTCCTCTACACATCTGACGCTGCCGACGAAGAGGA730.10602451635391855No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA690.10021495381397781No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCT690.10021495381397781No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTT108.4477826E-4295.01
TGCTTAT68350.0294.56841
CTTATAG67900.0293.913853
TTATAGA68100.0293.050664
GCTTATA68550.0292.84832
GAACAGG67500.0292.377789
TATAGAA69150.0287.747655
ATAGAAC69550.0284.396126
TAGAACA69550.0283.971957
AGAACAG70500.0279.936168
GCTTATG251.86943E-7236.02
GAGCAGG202.2728476E-5221.259
GAACCGG202.2728476E-5221.259
TATGGAA150.0028439062196.666675
GAAAGGC150.0028439062196.666679
CTTATGG150.0028439062196.666673
GAAAAGG457.6397555E-11196.666679
GCTTTTA150.0028439062196.666672
GCTTAAG255.533969E-5177.02
CTTAAAG200.006724041147.53
Produced by FastQC (version 0.11.5)