FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-178-6-1-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-178-6-1-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62244
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2539940.80553948975002No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT52998.513270355375619No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT30764.941841783947047No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG25424.083927768138294No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT21073.3850652271704904No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT19293.0990938885675727No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT10891.7495662232504336No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT8441.355953987532935No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT8201.3173960542381595No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT7951.2772315403894352No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT6170.9912602017865176No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT5810.9334233018443544No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT4680.7518796992481203No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT4660.7486665381402223No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA3590.5767624188676821No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG3450.5542702911123963No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT3450.5542702911123963No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT3310.5317781633571108No Hit
GAGACTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTGT3260.5237452605873658No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC3080.4948268106162843No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT2540.4080714607030396No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT2520.4048582995951417No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT2450.39361223571749887No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTGTTG2370.3807595912859071No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT2150.3454148190990296No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT2060.33095559411348885No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT2050.3293490135595399No Hit
GAGACTTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTG1900.3052503052503053No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCT1810.29079108026476447No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTG1670.2682989525094788No Hit
GAGACTTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCAG1550.24901998586209112No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1450.23295418032260135No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1390.22331469699890752No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG1390.22331469699890752No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTGTTG1290.20724889145941777No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT1290.20724889145941777No Hit
GAGACTTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTGTT1220.19600282758177495No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACT1130.18154360259623417No Hit
GAGACTTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG1070.17190411927254032No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT1060.17029753871859135No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT1050.16869095816464239No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCT1020.16387121650279546No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT980.15744489428699954No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTGTTGC940.15101857207120364No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCTAGGTCCT930.14941199151725468No Hit
GAGACTTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGT930.14941199151725468No Hit
GAGACTTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTGTTGC800.128526444315918No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA770.12370670265407108No Hit
GAGACTTAGAACAGGCTCCTCTGCATAAAAACACATAAAAACACGCGCGC680.10924747766853031No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTGTGT680.10924747766853031No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAGGCACTGCCAAGTCCT670.10764089711458133No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG650.10442773600668337No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT650.10442773600668337No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT640.1028211554527344No Hit
GAGACTTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTAT640.1028211554527344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTG157.207549E-6295.02
ACGCAGG108.4458233E-4295.0295
AGACCGA108.4458233E-4295.0295
GAACGGC206.1383616E-8295.09
GAACCGG108.4458233E-4295.09
GAGACTT61700.0294.521851
GACTTAG61150.0294.517583
AGACTTA61550.0294.041442
ACTTAGA61200.0294.035984
CTTAGAA61050.0294.033575
GAACAGG60700.0292.579
TTAGAAC61800.0288.79456
TAGAACA62450.0285.080087
AGAACAG63250.0281.47438
GACTTAT150.002843248196.666673
CTTGAAC150.002843248196.666675
GAAAAGG451.7662387E-8163.888899
AGGAGAA200.00672249147.5295
GACTTGA200.00672249147.53
TTGAACA200.00672249147.56
Produced by FastQC (version 0.11.5)