FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-177-6-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-177-6-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61330
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2334138.05804663296918No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT50938.304255666068808No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT37956.18783629545084No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT34685.654655144301321No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT18232.972444154573618No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC17722.889287461275069No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT13262.1620740257622697No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC12862.096853089841839No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT10601.728354801891407No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT7741.2620251100603295No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT7491.2212620251100603No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTC7071.1527800423936083No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTCATCCA6661.0859285830751673No Hit
GAGACTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTCATC6371.038643404532855No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT6130.9995108429805968No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT5690.9277678134681232No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTCATCCA5460.8902657753138757No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT5440.8870047285178543No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCC4930.8038480352193054No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTGGCGGGGTATCTAATCCCAGTC3620.5902494700798957No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT3270.5331811511495189No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT2890.47122126202511005No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT2760.45002445785097017No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT2720.44350236425892714No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT2640.430458177074841No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCATC2360.3848035219305397No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC2320.37828142833849665No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC2120.34567096037828143No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTT2090.34077939018424913No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATC2080.33914886678623835No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1950.31795206261209846No Hit
GAGACTTAGAACAGGCTCCTCGTCGGGGTATCTAATCCCAGTCATCCAGC1820.2967552584379586No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACAGCCAGGTCCT1780.29023316484591555No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1530.2494700798956465No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTACT1270.2070764715473667No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTCATCC940.15326919941301156No Hit
GAGACTTAGAACAGGCTCCTCTGACGCGCCGACGGGGTATCTAATCCCAG910.1483776292189793No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC900.14674710582096853No Hit
GAGACTTAGAACAGGCTCCTCTAAATGGAGGCAATTATTAAATTTGCAGT840.13696396543290398No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT840.13696396543290398No Hit
GAGACTTAGAACAGGCTCCTCGCATTAGCTGGGGTATCTAATCCCAGTCA790.12881134844285014No Hit
GAGACTTAGAACAGGCTCCTCCTACAGTAAGCTTTTTCCCGCTAGCTTCC790.12881134844285014No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT790.12881134844285014No Hit
GAGACTTAGAACAGGCTCCTCTGCCGCCAAAGGTTCATATGAGAAGATGG710.11576716125876406No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA680.11087559106473178No Hit
GAGACTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT640.10435349747268874No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA640.10435349747268874No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGGC108.4455253E-4295.09
AGACTTA60700.0294.513982
GACTTAG60350.0294.266753
CTTAGAA60300.0294.266175
GAGACTT61000.0293.790951
ACTTAGA60450.0293.779974
GAACAGG59500.0292.27319
TTAGAAC61950.0284.285746
TAGAACA62500.0281.076027
AGAACAG63550.0275.735668
GACTTAA450.0262.222233
GAAAAGG500.0206.59
ACTTGAA150.0028431483196.666664
CTTGAAC150.0028431483196.666665
TTAGAAA750.0177.06
AGACTTG352.1133487E-4126.4285662
CTTAAAC609.836913E-8122.916665
GACTAGA455.743853E-498.3333363
AAACAGG951.5188343E-677.6315848
GAGACTA806.0763803E-573.751
Produced by FastQC (version 0.11.5)