FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-176-6-4-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-176-6-4-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61193
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2637643.102969293873485No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT33145.415652117072215No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT25594.181850865295051No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT20653.3745689866487996No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT17632.8810484859379337No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT17032.782998055333126No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTG13902.2715016423447127No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTGT13662.2322814701027895No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT12302.0100338273985585No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT10231.671759841811972No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT9701.5851486281110585No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT8331.3612668115634141No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT5680.9282107430588465No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTTG4750.7762325756213946No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT4730.7729642279345676No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTCCTT4720.7713300540911542No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCCTT4570.7468174464399523No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCTAGGTCCTT4510.7370124033794715No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT4110.671645449642933No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGCC4040.6602062327390388No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT3760.6144493651234618No Hit
ATCTGTAGAACAGGCTCCTCTGGCCGTAAGCGATAAAACCATCCTGCGCC3450.5637899759776445No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCCT3320.5425457160132695No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT3110.5082280653015868No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG3040.49678884839769255No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTGT2720.4444952854084617No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAATCCTT2590.42325102544408677No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGCC2590.42325102544408677No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCCTT2520.41181180854019245No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCACCAAGTCCTT2400.39220172241923096No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCTG2370.38729920088899056No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGTTATGAAGCACCGCCAGGTCCTT2270.37095746245485595No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCCT2090.3415423332734136No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT1990.325200594839279No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1870.30559050871831744No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCTGGTCCTT1700.2778095533802886No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT1700.2778095533802886No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTTG1450.23695520729495206No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCTT1440.23532103345153854No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1430.2336868596081251No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGCC1400.22878433807788473No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT1390.22715016423447126No Hit
ATCTGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTTG1340.21897929501740396No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT1320.21571094733057705No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1320.21571094733057705No Hit
ATCTGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTGG1300.21244259964375012No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGGC1120.18302747046230777No Hit
ATCTGTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGTGGC1110.1813932966188943No Hit
ATCTGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGGCCT1070.17485660124524047No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAGT980.1601490366545193No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGCC980.1601490366545193No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTT950.15524651512427892No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGGC910.14870981975062508No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT870.14217312437697122No Hit
ATCTGTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTTGT820.1340022551599039No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC790.1290997336296635No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTGT790.1290997336296635No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGGGTATCTAATCCCAGTTGTGGC760.12419721209942314No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT700.11439216903894237No Hit
ATCTGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT700.11439216903894237No Hit
ATCTGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT700.11439216903894237No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTTG660.10785547366528853No Hit
ATCTGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTTGT650.10622129982187506No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGCC640.10458712597846159No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAAGTCTTT640.10458712597846159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG60450.0294.5122
ATCTGTA60600.0294.26981
CTGTAGA60300.0294.021553
AACAGGC59750.0291.54399
GAACAGG60050.0290.578678
TGTAGAA62350.0282.69854
GTAGAAC62100.0282.411445
TAGAACA62350.0280.805946
AGAACAG62700.0278.767947
ACAACGG150.0028431313196.66666295
TCTGAGA304.6344758E-7196.666662
CAAACTG150.0028431313196.66666295
CTGTAGG150.0028431313196.666663
ATCTGAG408.736606E-9184.3751
AAAGGCT255.530955E-5177.09
GACAGGC553.092282E-10160.909098
GCACGCG200.0067222132147.5295
TCTGTGA401.9420113E-6147.52
CAGCTAC200.0067222132147.5295
TACAGGC200.0067222132147.59
Produced by FastQC (version 0.11.5)