FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-175-6-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-175-6-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56534
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT3738166.1212721548095No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT21193.7481869317578806No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT19383.428025612905508No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT14462.5577528566880106No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT13692.4215516326458415No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT11562.0447872076980222No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT10511.859058265822337No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT6801.2028160045282486No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT5350.9463331800332544No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT4850.8578908267591183No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT3160.5589556726925391No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT2780.4917394842041957No Hit
CTAGGTAGAACAGGCTCCTCTAGCCAGAATCTTGTAAAAAAATCTGCGCT2390.4227544486503697No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTACTT1630.2883220716736831No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1610.2847843775427177No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCCTT1470.2600205186259596No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCTTT1370.24233204797113242No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT1190.21049280079244348No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCCTT1110.19634202426858172No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCTT1110.19634202426858172No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACAGCCAAGTCCTT1000.17688470654827185No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT980.1733470124173064No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG980.1733470124173064No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT970.17157816535182369No Hit
CTAGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT960.16980931828634097No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCCT920.16273393002441008No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT910.1609650829589274No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT870.1538896946969965No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAAGTCCTT870.1538896946969965No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGATATGAAGCACCGCCAGGTCCTT800.14150776523861747No Hit
CTAGGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT770.1362012240421693No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC760.1344323769766866No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT690.12205044751830757No Hit
CTAGGAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT660.11674390632185941No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCGT650.11497505925637669No Hit
CTAGGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT620.10966851805992853No Hit
CTAGGTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT570.10082428273251495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTT255.2386895E-10295.01
CTAGGTA55850.0295.01
GTTAGAA255.2386895E-10295.05
AGGTCGA108.443777E-4295.03
AGGTTAG255.2386895E-10295.03
GGTATAA108.443777E-4295.04
TAGGTTA255.2386895E-10295.02
GGTCGAA108.443777E-4295.04
TAGGTCG108.443777E-4295.02
TAGGTAT108.443777E-4295.02
TAGGTAG55500.0295.02
GGTAGAA55350.0294.200564
AGGTAGA55650.0293.93983
GAACAGG55400.0292.07138
AACAGGC55550.0291.017129
GTAGAAC56200.0288.701055
AGAACAG57250.0282.375557
TAGAACA57750.0280.441566
GGTAGAC251.8664832E-7236.04
GGTGAAC202.2706958E-5221.254
Produced by FastQC (version 0.11.5)