FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-174-6-4-8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-174-6-4-8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56032
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2758649.232581382067394No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT48748.698600799543119No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT22944.094089091947459No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT20693.6925328383780696No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT15732.8073243860651056No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCACT12922.3058252427184467No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT8991.6044403198172474No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT8481.513420902341519No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT6901.2314391776127926No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT6071.083309537407196No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT5540.9887207310108509No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT4190.7477869788692175No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT3980.7103083952027414No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT3640.6496287835522558No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT3180.5675328383780697No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG2510.44795830953740723No Hit
AGGAATAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTCAA2340.41761850371216447No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAATCA2120.37835522558537976No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCTT2080.37121644774414625No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT1970.3515848086807538No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT1890.3373072529982867No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTC1820.3248143917761279No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT1720.306967447173044No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATCAC1680.2998286693318104No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT1540.2748429468874929No Hit
AGGAATAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCCTT1490.2659194745859509No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCAAT1350.24093375214163334No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT1320.23557966876070818No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT1110.19810108509423188No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCAATC1070.1909623072529983No Hit
AGGAATAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGTC1050.1873929183323815No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT980.17490005711022272No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCTT980.17490005711022272No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCTT940.16776127926898915No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT930.16597658480868074No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT870.1552684180468304No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT820.14634494574528842No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT800.1427755568246716No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT790.14099086236436323No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT780.13920616790405482No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT780.13920616790405482No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAATC760.13563677898343804No Hit
AGGAATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTCAATC730.13028269560251285No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG680.12135922330097086No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCCT660.11778983438035409No Hit
AGGAATAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTCAAT640.1142204454597373No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT640.1142204454597373No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT620.1106510565391205No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAATC610.10886636207881212No Hit
AGGAATAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTATT570.10172758423757852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGG108.4435777E-4295.0295
AACAGGC54800.0293.385049
AGGAATA56100.0292.107851
GAACAGG55100.0291.519968
GAATAGA56750.0287.98243
GGAATAG57050.0286.468022
ATAGAAC56750.0285.123355
TAGAACA56750.0284.08376
AGAACAG57500.0279.60877
AATAGAA58850.0277.706024
CTCAACG150.0028424938196.66667295
GAAAGGC150.0028424938196.666678
GAGCGCA150.0028424938196.66667295
GCGCTAG150.0028424938196.66667295
GAAAAGG501.6007107E-10177.08
GAAGAGG200.006720711147.5295
AAAAGGC503.3096512E-8147.59
TAACAGG200.006720711147.58
CGAGCCA200.006720711147.5295
AGGAAAG453.4871464E-6131.111111
Produced by FastQC (version 0.11.5)