FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-173-6-1-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-173-6-1-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55653
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2338542.019298151043074No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT53449.60235746500638No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT29055.219844392934792No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT23254.1776723626758665No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT17383.1229223941207125No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGTC16682.997143011158428No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT9981.7932546313765656No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT7311.313496127791853No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT6641.1931072898136668No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT6491.1661545648931773No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT6371.1445923849567858No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT5450.9792823387777838No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCTT5330.9577201588413922No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT3980.715145634556987No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT3550.6378811564515839No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTGT3220.5785851616265071No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCTG3110.5588198300181482No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCTT2840.5103049251612671No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG2160.38811923885504823No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTACC2130.3827286938709503No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCTTT2050.3683539072466893No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT1820.3270263957019388No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTGT1770.31804215406177566No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGTA1610.28929258081325354No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACCC1590.2856988841571883No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCCT1520.27312094586095986No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCTT1500.2695272492048946No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT1470.2641367042207967No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT1460.2623398558927641No Hit
CTAGGTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTATT1360.24437137261243777No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT1350.24257452428440518No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCTT1320.23718397930030727No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT1300.233590282644242No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT1230.2210123443480136No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT1230.2210123443480136No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCCTT1080.19405961942752412No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACCCT1020.18327852945932832No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT1010.1814816811312957No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTGT1000.17968483280326308No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT980.17609113614719782No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCTT950.1707005911630999No Hit
CTAGGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTTG940.1689037428350673No Hit
CTAGGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTGT930.16710689450703467No Hit
CTAGGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTAC910.1635131978509694No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT900.1617163495229368No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCCTG870.15632580453883888No Hit
CTAGGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGTA870.15632580453883888No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT860.15452895621080623No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCCT830.14913841122670835No Hit
CTAGGTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATCTAA820.14734156289867573No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACCCT800.14374786624261046No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTG780.1401541695865452No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCAT780.1401541695865452No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTCCTT770.13835732125851258No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT760.13656047293047993No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCCT750.1347636246024473No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT740.13296677627441467No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTCCTT720.12937307961834943No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGAATATGAAGCACCGCCAGGTCCTT640.11499829299408837No Hit
CTAGGTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTGTA600.10781089968195784No Hit
CTAGGTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTGTA590.10601405135392522No Hit
CTAGGTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGGT580.10421720302589259No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTG560.10062350636982732No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT560.10062350636982732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGG108.443436E-4294.99997295
AACCGGC108.443436E-4294.999979
TAGGTTA206.132541E-8294.999972
GAAAGGC206.132541E-8294.999978
GAACCGG108.443436E-4294.999978
GGTAGGA108.443436E-4294.999974
CTAGGTA55150.0294.46511
TAGGTAG54750.0294.19182
AGGTAGA54750.0293.652983
GGTAGAA54700.0293.382084
GAACAGG54600.0291.217968
AACAGGC54700.0290.685559
TAGAACA56400.0282.44686
AGAACAG56300.0282.42457
GTAGAAC57000.0280.255
CTAGGTT301.5625119E-9245.833341
TAGGTAA403.274181E-11221.249982
GGTAAAC353.927198E-9210.714284
TAGGTGA353.927198E-9210.714282
ACTGCGA150.002842446196.66667295
Produced by FastQC (version 0.11.5)