FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-172-6-7-4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-172-6-7-4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51131
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC3073960.11812794586454No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC32426.340576167100194No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC27915.458528094502356No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC15212.9747120142379377No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC14402.816295398095089No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC12062.358647395904637No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC8361.635015939449649No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT5431.0619780563650232No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC4460.872269269132229No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC4100.8018618841798518No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC2600.5084977802116133No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA2100.4107097455555338No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTTGACCGA1710.33443507852379184No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC1690.33052355713754866No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC1590.3109659502063327No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC1540.30118714674072483No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC1480.28945258258199524No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTCTGAAGCACCGCCAAGTCCT1460.28554106119575207No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCC1360.2659834542645362No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC1320.2581604114920498No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC1280.25033736871956347No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC1190.23273552248146917No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT1130.22100095832273964No Hit
CCACGTCTAGAACAGGCTCCTCTACTTGTGCGGGTCCCCGTCTATTCCTT1040.20339911208464534No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCTAGTCC920.17992998376718625No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT800.15646085544972718No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC790.1545050947566056No Hit
CCACGTCTAGAACAGGCTCCTCTCCTTTCAAAGAGGATGCAGTATGGTTT750.1466820519841192No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGAC710.13885900921163286No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC700.1369032485185113No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC640.12516868435978173No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT590.11538988089417378No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCA590.11538988089417378No Hit
CCACGTTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC560.10952259881480902No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT540.10561107742856585No Hit
CCACGTCTAGAACAGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT530.10365531673544426No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC530.10365531673544426No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT520.10169955604232267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGA108.441417E-4295.0295
ACGTCGA108.441417E-4295.03
CCACGTT303.6379788E-12295.01
CACGTTC108.441417E-4295.02
CACGTTA206.1276296E-8295.02
GTTCTAG108.441417E-4295.05
CGTTCTA108.441417E-4295.04
CCACGTC50800.0294.709661
GTCTAGA50150.0294.705875
CGTCTAG50150.0294.705874
CACGTCT50450.0294.415252
ACGTCTA50400.0293.829353
CTAGAAC50100.0292.939127
TCTAGAA50750.0290.05916
TAGAACA51350.0284.65928
AGAACAG51800.0281.901559
CGTTAGA251.8647552E-7236.04
TCTAGAC251.8647552E-7236.06
ACGTTAG251.8647552E-7236.03
CGTCAGA353.921741E-9210.714284
Produced by FastQC (version 0.11.5)