FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-171-6-2-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-171-6-2-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50518
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2965658.703828338414034No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC31776.288847539490875No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC29275.793974424957441No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC25535.053644245615424No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC9251.8310305237737043No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC7661.5162912229304406No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC7461.476701373767766No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC5871.1619620729245022No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC5391.066946434934083No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT4600.9105665307415179No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTAC4140.819509877667366No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC3740.7403301793420167No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGG3520.6967813452630746No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA2660.5265449938635733No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA2550.5047705768241023No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTG2460.4869551447008987No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGG2140.4236113860406192No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1950.3860010293360782No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCT1600.3167187933013975No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT1310.2593135120155192No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGG1210.23951858743418186No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTG1160.2296211251435132No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC1120.22170315531097828No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTG900.1781543212320361No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT750.1484619343600301No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT720.1425234569856289No Hit
CCACGTCAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT600.11876954748802407No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT580.1148105625717566No Hit
CCACGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT570.11283107011362287No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCCCCGCCAGGTCC560.11085157765548914No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGCCC530.10491310028108794No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC530.10491310028108794No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCAAGGTCC530.10491310028108794No Hit
CCACGTCTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC520.1029336078229542No Hit
CCACGTTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC510.10095411536482048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTT550.0295.01
CACGTTC206.126902E-8295.02
ACGTTCT206.126902E-8295.03
ACGTTAG350.0295.03
CCACGGC255.2386895E-10295.01
CCACGTC49400.0294.701421
CGTCTAG48850.0294.698064
GTCTAGA48900.0294.396735
CACGTCT49400.0294.104252
ACGTCTA49450.0293.80693
CTAGAAC49000.0293.193887
TCTAGAA49450.0289.929236
TAGAACA50250.0285.606968
AGAACAG51200.0280.307629
CGTTAGA400.0258.1254
GTTAGAA400.0258.1255
GGCTAGA251.8645369E-7236.05
CGTTCTA251.8645369E-7236.04
CGTCTAA600.0221.249984
CACGGCT353.921741E-9210.714282
Produced by FastQC (version 0.11.5)