FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-170-6-1-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-170-6-1-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49308
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2271946.07568751521051No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC38607.828344284903058No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC21274.313701630567048No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC19984.052080798247749No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC14742.989372921229821No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG13792.7967064168086315No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC9351.8962440171980206No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC8761.776587977610124No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC5301.074876287823477No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT4810.9755009329114951No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC4390.8903220572726535No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC3690.7483572645412508No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCC3530.7159081690597874No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC3360.6814310051107325No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT2830.5739433763283849No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCC2640.535410075444147No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG2490.504989048430275No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA2410.48876450068954325No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT2160.4380627889997567No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT1880.3812768719071956No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCC1860.37722073497201264No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC1810.36708039263405534No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACC1690.3427435710229577No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTA1660.33665936562018334No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT1540.3123225440090857No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC1490.3021822016711284No Hit
GCGAAGTTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGT1280.25959276385170765No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC1190.24134014764338446No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC1160.23525594224061003No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC1150.23322787377301857No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC1140.2311998053054271No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC1140.2311998053054271No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC1100.22308753143506127No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG1070.21700332603228684No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC1020.20686298369432954No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC980.19875070982396364No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCC980.19875070982396364No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACC920.18658229901841486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTAC900.1825261620832319No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCC870.17644195668045753No Hit
GCGAAGTTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTT870.17644195668045753No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCC860.17441388821286605No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT790.1602174089397258No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT770.15616127200454286No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC720.14602092966658553No Hit
GCGAAGTTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTA710.14399286119899407No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCC690.13993672426381115No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACC680.13790865579621966No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCC670.1358805873286282No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC660.13385251886103675No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTCC660.13385251886103675No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC630.12776831345826234No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGTG610.12371217652307942No Hit
GCGAAGTTAGAACAGGCTCCTCTTAGCGTCAGGGGTATCTAATCCCAGTG600.12168410805548796No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGTCTCGTGGGGTATCTAATCCCA590.1196560395878965No Hit
GCGAAGTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT560.1135718341851221No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC540.10951569724993918No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC540.10951569724993918No Hit
GCGAAGTTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT530.10748762878234769No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT530.10748762878234769No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCC510.10343149184716477No Hit
GCGAAGTTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCC500.1014034233795733No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGG108.4405154E-4295.09
CACACGG108.4405154E-4295.0295
AAGTTGA157.1984923E-6294.999974
GCGAAGT49100.0294.69961
GTTAGAA48050.0294.693026
CGAAGTT48900.0294.396732
GAAGTTA48700.0294.394263
AAGTTAG48600.0294.08954
AGTTAGA48500.0293.78355
TTAGAAC48900.0289.268927
TAGAACA49950.0283.18828
AGAACAG50300.0281.217689
GAAGTTG251.8640822E-7236.03
AGTTGAA202.2689446E-5221.255
TTGAACA202.2689446E-5221.257
GTTAGAC353.919922E-9210.714286
GCGAAAT200.006718286147.51
TCATGTG200.006718286147.5295
AACGAGG200.006718286147.5295
TTAGACA505.8818787E-6118.07
Produced by FastQC (version 0.11.5)