FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-17-6-4-8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-17-6-4-8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11554
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC584450.579885753851485No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC10899.42530725289943No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATC3593.107149039293751No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3563.0811840055392072No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC3282.8388436904967973No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC3202.7696036004846807No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTC2692.3281980266574345No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG2622.2676129478968323No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC2572.224337891639259No Hit
AATCCTATTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT2342.025272632854423No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGACAGTCTCTCGCCAGGGGTATC1851.601177081530206No Hit
AATCCTATTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG1811.5665570365241477No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCT1511.3069066989787086No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC1341.15977150770296No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1261.090531417690843No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1191.0299463389302406No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC990.8568461138999481No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCA910.7876060238878311No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA810.7010559113726847No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTC420.36351047256361435No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCA260.22503029253938028No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCA210.18175523628180718No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC210.18175523628180718No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC190.1644452137787779No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCA180.15579020252726328No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC140.12117015752120479No Hit
AATCCTATTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTC130.11251514626969014No Hit
AATCCTATTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC120.10386013501817552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC11350.0295.08
ATCCTAT11500.0295.02
CCTATTA11400.0295.04
CCTATAG108.3570444E-4295.04
TCCTATT11400.0295.03
TCCTATA108.3570444E-4295.03
TATTAGA11400.0295.06
CTATTAG11400.0295.05
AATCCTA11550.0293.722961
ATTAGAA11500.0291.15227
TAGAACA11550.0287.337689
CTATAGA150.002813437196.666665
TATCGTA150.002813437196.66666295
TGTTGAG6150.059.000004105-109
CCGCCAG6600.059.00000440-44
ATGGCTC452.5465852E-1159.00000465-69
ATGAAGC6650.059.00000430-34
GGCGAGC6600.059.00000480-84
GCTATGG452.5465852E-1159.00000460-64
GCCAGGT6600.059.00000440-44
Produced by FastQC (version 0.11.5)