FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-169-6-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-169-6-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48475
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1631133.64827230531201No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT526210.855079938112429No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT28135.80299123259412No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT24204.992264053635894No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT21614.457968024755028No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT17863.6843733883445076No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC14933.0799381124290868No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT9752.0113460546673543No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT7481.5430634347601857No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT7441.5348117586384733No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT5611.1572975760701392No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT4981.0273336771531718No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCA4670.963383187209902No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG4170.8602372356884993No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT4040.8334192882929345No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGGGTATCTAATCCCAGTCATC3640.7509025270758123No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTCATCC3580.738525012893244No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA3520.7261474987106756No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT3210.6621970087674058No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCT2490.5136668385765859No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA2470.5095410005157297No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCCAGGGGGTATCTAATCCCAGTCA2450.5054151624548736No Hit
GAGTGGTAGAACAGGCTCCTCTAGGAGAGGGAGAGAGGGGGTATCTAATC2410.49716348633316143No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGAGGGGTATCTAATCCCAGTCATCC2290.4724084579680247No Hit
GAGTGGTAGAACAGGCTCCTCCATTCCCAGGGTATCTAATCCCAGTCATC2190.4517792676637442No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTCATCCA2170.4476534296028881No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT2150.44352759154203203No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGGGGTATCTAATCCCAGTCAT2110.43527591542031974No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA2100.4332129963898917No Hit
GAGTGGTAGAACAGGCTCCTCTAGCCCCGGGGGGTATCTAATCCCAGTCA2040.42083548220732336No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCTCAGGGGTATCTAATCCCAGTCA2010.41464672511603917No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA1680.34657039711191334No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA1680.34657039711191334No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT1340.27643115007735947No Hit
GAGTGGTAGAACAGGCTCCTCTACACATCCGGGGGGTATCTAATCCCAGT1300.26817947395564723No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCT1150.23723568849922638No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGTATCTAATCCCAGTCATCCAGCTG1060.2186694172253739No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT940.19391438886023724No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCT890.18359979370809695No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT870.17947395564724083No Hit
GAGTGGTAGAACAGGCTCCTGGGGGTATCTAATCCCAGTCATCCAGCTGT840.17328519855595667No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA730.15059308922124806No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT700.1444043321299639No Hit
GAGTGGTAGAACAGGCTCCTCTCAAAGCAGATTTAGACACCTGCATTATG640.13202681794739557No Hit
GAGTGGTAGAACAGGCTCCTCTGTCTCTTATACACATCTGACGCTGCCAA620.12790097988653945No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT590.12171222279525529No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGTGGGGGTATCTAATCCCAGTCATC570.11758638473439917No Hit
GAGTGGTAGAACAGGCTCCTCTACCAGCTCTGAAGAACGCTCCAGAAACT510.10520887055183084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGC108.4400654E-4295.09
GAGTGGT47900.0294.384161
AGTGGTA47800.0294.382842
TGGTAGA47700.0294.381564
GGTAGAA47650.0293.76185
GAACAGG47350.0292.19649
GTGGTAG48300.0290.724643
GTAGAAC48200.0288.879676
TAGAACA48950.0283.850867
AGAACAG49550.0279.818368
GACAGGC150.0028413148196.666679
AACCAGG150.0028413148196.66667295
CGCTCAA150.0028413148196.66667295
CCAACCG150.0028413148196.66667295
AGAACGG200.0067179296147.58
GTGGTAA200.0067179296147.53
GAGTGGA352.110636E-4126.4285661
GAAAAGG352.110636E-4126.4285669
GTAGAAA609.805444E-8122.916676
AAGTCGA550.001273224780.454544295
Produced by FastQC (version 0.11.5)