FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-168-6-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-168-6-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48120
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC2781057.793017456359095No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC32926.841230257689111No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC31336.510806317539484No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC14112.932252701579385No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC11102.306733167082294No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC9692.013715710723192No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC7381.5336658354114712No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC7011.4567747298420615No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA4350.9039900249376559No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC4030.8374896093100582No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTC3980.827098919368246No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2930.6088944305901912No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTC2090.4343308395677473No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTTG2070.43017456359102246No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT2040.4239401496259352No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAGGCACTGCCAAGTC2020.4197838736492103No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTG1760.3657522859517872No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1660.34497090606816294No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG1470.3054862842892768No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC1430.2971737323358271No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC1180.24522028262676643No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1120.23275145469659184No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTTG1090.22651704073150458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC1090.22651704073150458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT990.2057356608478803No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC990.2057356608478803No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA930.19326683291770574No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGAGAGGGGTATCTAATCCCAGTTG920.19118869492934332No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGT840.1745635910224439No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG810.16832917705735662No Hit
TTCTCAGCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC720.14962593516209477No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAG680.14131338320864506No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTC610.12676641729010807No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGATATGAAGCACCGCCAGGTC560.11637572734829592No Hit
TTCTCAGCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC550.1142975893599335No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC530.11014131338320864No Hit
TTCTCAGCTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC500.10390689941812137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG157.197401E-6295.04
CTCGCTA157.197401E-6295.03
TCTCAGC47250.0294.687842
CTCAGCT47000.0294.686163
TCAGCTA46950.0294.05754
CAGCTAG46650.0293.735265
TTCTCAG47800.0293.148561
AGCTAGA46700.0292.473246
GCTAGAA46750.0291.529427
CTAGAAC46600.0290.568668
TAGAACA48350.0279.441569
GCTAGAC251.8636092E-7236.07
CGCTAGA202.2686017E-5221.255
TCTCGCT202.2686017E-5221.252
TTCTCGC202.2686017E-5221.251
GCTGAAC150.0028412512196.666677
TCTCTGC150.0028412512196.666672
ACGCGAA150.0028412512196.66667295
CTCAGCA408.707502E-9184.3753
CAGCTAA501.5825208E-10177.05
Produced by FastQC (version 0.11.5)