FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-167-6-8-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-167-6-8-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47875
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC2245746.907571801566576No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC34007.101827676240209No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC23844.979634464751958No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCT21654.522193211488251No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC16893.527937336814621No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC10022.0929503916449086No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC7911.6522193211488252No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC6291.3138381201044387No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC5311.1091383812010442No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC4780.9984334203655352No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCC3670.766579634464752No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACC3490.7289817232375979No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT2890.6036553524804178No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG2860.5973890339425587No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC2800.5848563968668407No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC2720.5681462140992166No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTC2670.5577023498694517No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC2450.5117493472584856No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGT2330.4866840731070496No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTC2090.4365535248041776No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGT1900.3968668407310705No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCC1880.3926892950391645No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTA1880.3926892950391645No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTAC1830.3822454308093995No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC1580.33002610966057444No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC1540.3216710182767624No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTAC1330.27780678851174934No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTAC1210.25274151436031334No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC1150.2402088772845953No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC1130.2360313315926893No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC1110.23185378590078332No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC1010.21096605744125324No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG970.20261096605744122No Hit
TCTCAATCTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCT960.20052219321148823No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG940.19634464751958225No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC900.18798955613577023No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTAC890.18590078328981724No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGGGGCACCGCCAAGTC860.17963446475195824No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA860.17963446475195824No Hit
TCTCAATCTAGAACAGGCTCCTCTCGTGGGGTATCTAATCCCAGTGTACC830.1733681462140992No Hit
TCTCAATCTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGG830.1733681462140992No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTA750.1566579634464752No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC710.14830287206266318No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC680.14203655352480415No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA660.13785900783289817No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC620.12950391644908618No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAGGTC600.12532637075718017No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTC580.12114882506527415No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC570.11906005221932114No Hit
TCTCAATCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC570.11906005221932114No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA560.11697127937336815No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTC560.11697127937336815No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC550.11488250652741513No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTC520.10861618798955613No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTC520.10861618798955613No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGGGTATCTAATCCCAGTGTAC520.10861618798955613No Hit
TCTCAATCTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGT510.10652741514360314No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGAGGGGTATCT500.10443864229765012No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTC490.10234986945169713No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTC480.10026109660574412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAA108.439739E-4295.0295
GGTAGCA108.439739E-4295.0295
CTCATCT303.6379788E-12295.02
CTCAATC47300.0294.688142
TCAATCT47200.0294.3753
AATCTAG47050.0293.432535
CAATCTA47500.0291.894744
ATCTAGA47350.0290.950386
TCTCAAT48100.0290.40021
CTAGAAC47450.0288.782938
TCTAGAA47750.0287.58647
TAGAACA48650.0281.659829
TCTCAAC202.268528E-5221.251
TCTGAAC150.002841205196.666677
CATCTAG457.6397555E-11196.666664
TCATCTA501.5825208E-10177.03
TCTCATC501.5825208E-10177.01
GAGAGCG200.006717671147.5295
CTCAACT200.006717671147.52
CCGACAA403.5906734E-4110.625295
Produced by FastQC (version 0.11.5)