FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-166-6-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-166-6-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46539
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2046643.976020112163994No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT43619.370635381078236No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT22324.795977567201701No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT21264.568211607469005No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT14603.137153784997529No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG13262.8492232321278927No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT11752.524764176282258No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT6781.4568426481015921No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT6151.3214723135434796No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA5651.21403554008466No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC2880.6188358151228002No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTTGTGGCC2750.5909022540235072No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2740.5887535185543308No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2690.5780098412084488No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT2570.5522250155783321No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT2560.5500762801091558No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTGTAAAGCACCGCCAAGTCCT2360.5071015707256279No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2300.49420915791056963No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAATCCT2230.47916800962633493No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT2160.4641268613421002No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT2090.4490857130578655No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCC2020.43404456477363074No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCT1980.4254496228969252No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT1720.36958250069833903No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT1600.34379767506822234No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGACAGGTCCT1580.3395002041298696No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT1520.3266077913148112No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1490.3201615849072821No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGTG1470.31586411396892927No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT1450.3115666430305765No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG1430.3072691720922237No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCT1410.30297170115387095No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC1350.29007928833881264No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA1300.27933561099293064No Hit
GAGTGGTAGAACAGGCTCCTCTAGCATCGACGAGGGGTATCTAATCCCAG1280.2750381400545779No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGGGGTATCTAATCCCAGTTGT1280.2750381400545779No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTTGTGGC1220.26214572723951957No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGGTATCTAATCCCAGTTGTGG1210.2599969917703432No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1200.25784825630116676No Hit
GAGTGGTAGAACAGGCTCCTCTACAGGGGTATCTAATCCCAGTTGTGGCC1140.24495584348610844No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG1060.2277659597326973No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCC920.19768366316422784No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG890.19123745675669868No Hit
GAGTGGTAGAACAGGCTCCTCTCTCTCTCTCTCTCGGGGTATCTAATCCC850.1826425148799931No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGAGACGGGGTATCTAATCCCAGTTG850.1826425148799931No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT770.165452631126582No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT750.16115516018822923No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG690.14826274737317088No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTTGT680.1461140119039945No Hit
GAGTGGTAGAACAGGCTCCTCTACAGGGATGTAAAGCACCGCCAAGTCCT620.13322159908893616No Hit
GAGTGGTAGAACAGGCTCCTCTAGGAGGGGGGTATCTAATCCCAGTTGTG610.13107286361975978No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT550.11818045080470142No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCCGGGTATCTAATCCCAGTT520.11173424439717225No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTT108.438973E-4295.02
GAAAAGG157.195862E-6294.999979
GAGTGGT45900.0294.678651
AGTGGTA45750.0294.03282
GGTAGAA45350.0293.373755
TGGTAGA45700.0292.417944
GAACAGG45350.0289.796029
GTGGTAG46200.0289.253273
GTAGAAC45700.0288.86766
AGAACAG47100.0280.28138
TAGAACA47450.0277.903087
GTGGTAA301.5570549E-9245.833313
GGTAGAC202.268117E-5221.255
GGTAAAA150.0028409483196.666665
AGTGGAA150.0028409483196.666662
AGAACCG150.0028409483196.666668
TGGTAAA304.622616E-7196.666664
GGTGAAC255.5224707E-5177.05
AGAGAGG255.5224707E-5177.0295
GAGTGGA359.963278E-7168.571431
Produced by FastQC (version 0.11.5)