FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-165-6-4-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-165-6-4-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46249
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1960942.39875456766633No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT34907.546109105061731No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT22944.9601072455620665No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT19284.168738783541266No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT10612.2941036562952712No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT10202.205453090877641No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT8261.7859845618283638No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC7891.7059828320612338No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG6211.3427317347402106No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT5621.2151614088953275No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCT5401.1675928128175745No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT5281.1416463058660726No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCCT5181.1200242167398213No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT4440.9600207572055612No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT4060.877856818525806No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTCCT3880.8389370580985535No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGATCTAAAGCACCGCCAAGTCCT3760.8129905511470519No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT3580.7740707907197993No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT3230.6983934787779195No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT2940.6356894203117905No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCAT2550.5513632727194102No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT2540.549201063806785No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTG2460.5319033925057839No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT2440.5275789746805337No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT2250.486497005340656No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGATCCT2130.46055049838915435No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCT2070.4475772449134035No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCAAGGTCCT2030.438928409262903No Hit
TGCTTATAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC2000.4324417825250276No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT1980.4281173646997773No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTTGTGGC1860.40217085774827566No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT1690.3654133062336483No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT1640.3546022616705226No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT1610.3481156349326472No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT1470.31784471015589527No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCACCAAGTCCT1460.31568250124327013No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1360.2940604121170187No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT1200.2594650695150165No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT1060.2291941447382646No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCTGATGCATCTGGGTAATCTAAAT1040.22486972691301435No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT1020.22054530908776404No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCC1000.2162208912625138No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC960.20757205561201322No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC960.20757205561201322No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTCTAAGGCACCGTCAGGTCCT890.19243659322363726No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC890.19243659322363726No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG830.17946333974788645No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATGTTAAGCACCGCCAAGTCCT820.1773011308352613No Hit
TGCTTATAGAACAGGCTCCTCTAGACGACGACGACGACGAGACGGGGTAT730.15784125062163507No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTTGTGG710.15351683279638478No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGGCC710.15351683279638478No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGCTCGGGGTATCTAATCCCAGTTG690.1491924149711345No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCGCCGCCAAGTCCT670.14486799714588425No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG630.13621916149538368No Hit
TGCTTATAGAACAGGCTCCTCTAGCACTAAGGGCTCTTGGAGAACCGAAG610.13189474367013343No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC600.12973253475750826No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGTT580.125408116932258No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG570.12324590801963285No Hit
TGCTTATAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT540.11675928128175744No Hit
TGCTTATGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT520.11243486345650718No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA490.10594823671863177No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG490.10594823671863177No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT480.10378602780600661No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAGTT470.10162381889338148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG108.438797E-4295.02
TGCTTAT45950.0294.6791
GCTTATA45700.0294.677252
CTTATAG45650.0294.030673
TTATAGA45750.0293.065554
GAACAGG45400.0291.42629
TATAGAA46400.0287.688575
ATAGAAC46650.0285.830666
TAGAACA46900.0283.363537
AGAACAG47350.0280.670538
GCTTATG251.862827E-7236.02
GACAGGC202.2680224E-5221.259
CTTAAGA150.0028408892196.666663
GACACGC150.0028408892196.66666295
GAACGGC255.522241E-5177.09
TGCTTAA200.006716925147.51
CTTATGA401.9369218E-6147.53
TTAAGAA200.006716925147.54
ATAACTG200.006716925147.5295
TTATGAA301.1420062E-4147.499984
Produced by FastQC (version 0.11.5)