FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-164-6-7-4-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-164-6-7-4-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45727
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2195348.00883504275373No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC38458.408598858442495No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCC33427.308592297767183No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC21504.701817307061473No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC18694.087300719487392No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC17433.8117523563758833No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCC7911.7298313906444769No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTT5691.244341417543246No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCC3980.8703829247490542No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC3860.8441402235003389No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3100.6779364489251427No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC2750.6013952369497233No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT2150.4701817307061473No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT2090.45706038008178973No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC1850.4045749775843594No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGACATGAAGCACCGCCAGGTCC1820.39801430227218054No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC1740.3805191681063704No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCC1620.35427646685765524No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTCC1450.3170993067553087No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCT1380.30179106436022485No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAAGTTT1360.2974172808187723No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAG1320.28866971373586725No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCC1310.2864828219651409No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCGCCGCCAAGTCC1300.2842959301944147No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTCC1160.253679445404247No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC1120.2449318783213419No Hit
GCGAAGTTAGAACAGGCTCCTCTGCTTGTGCGGGTCCCCGTCTATTCCTT1080.2361843112384368No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC970.21212850176044787No Hit
GCGAAGTTAGAACAGGCTCCTCTACTTGTGCGGGTCCCCGTCTATTCCTT950.20775471821899535No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC940.20556782644826904No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGATATAAAGCACCGCCAAGTCC930.20338093467754279No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGAC910.19900715113609027No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT880.19244647582391147No Hit
GCGAAGTTAGAACAGGCTCCTCTGGTTTCCCAAAGGATGCAGCATGGTTT730.15964309926301748No Hit
GCGAAGTTAGAACAGGCTCCTCTCCTTTCAAAGAGGATGCAGTATGGTTT660.14433485686793363No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTTGAC640.13996107332648108No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACCACCTACAGCCTGTAGGGCAAT630.1377741815557548No Hit
GCGAAGTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT600.131213506243576No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCC570.1246528309313972No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTTGAC560.12246593916067094No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT550.12027904738994466No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT540.1180921556192184No Hit
GCGAAGTTAGAACAGGCTCCTCTATAGGTATAACCTCCTCTGTAGATCTC520.11371837207776586No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTTGACCGA520.11371837207776586No Hit
GCGAAGTTAGAACAGGCTCCTCTGGGGGGGTCTGAAGCACCGCCAAGTCC480.1049708049948608No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT460.10059702145340826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTTG108.438499E-4295.03
ATACGGA108.438499E-4295.0295
AAGTTGA108.438499E-4295.04
GCGAAGT45650.0294.67691
CGAAGTT45500.0294.67582
GTTAGAA45100.0294.672946
GAAGTTA45450.0294.350923
AAGTTAG45400.0293.700444
AGTTAGA45400.0293.375555
TTAGAAC46000.0288.586987
TAGAACA46700.0283.629558
AGAACAG47400.0278.818579
CTTTATA150.0028407883196.66667295
ATGAATG150.0028407883196.66667295
AGTTGAA150.0028407883196.666675
TTTCCCA200.006716689147.5295
GCTGCCC200.006716689147.5295
GTTAGAC200.006716689147.56
CAGTGGG200.006716689147.5295
CAGTGCA200.006716689147.5295
Produced by FastQC (version 0.11.5)