FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-163-6-1-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-163-6-1-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43715
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT2011946.023104197643825No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT33317.6198101338213435No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT23735.428342674139311No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT17073.9048381562392773No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT11652.6649891341644745No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTGT11082.534599107857715No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT9522.177742193755004No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCT9502.1731671051126615No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT4851.109458995768043No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT4190.9584810705707423No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT3770.862404209081551No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT3530.8075031453734416No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT3410.7800526135193869No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG3140.718288916847764No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGTTTGGGGCACCGCCAAGTCCT2790.6382248656067712No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT2690.6153494223950589No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT2530.5787487132563193No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTGTAC1890.4323458767013611No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTGT1850.42319569941667623No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTACCCT1380.31568111632162876No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCGCCGCCAGGTCCT1310.29966830607343015No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCTG1250.2859430401464028No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCT1160.26535514125586185No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGT1030.23561706508063593No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA1020.23332952075946473No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTACC990.22646688779595103No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCCT930.2127416218689237No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTACCC870.1990163559418964No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTCCT860.19672881162072514No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT840.1921537229783827No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCT740.16927827976667048No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT720.16470319112432802No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACGGGGGGGTATCTAATCCCAGTGT670.15326546951847192No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCT660.15097792519730072No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG650.14869038087612949No Hit
GAGTGGTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTGTA650.14869038087612949No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTCCT630.14411529223378705No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCT630.14411529223378705No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACCC620.14182774791261582No Hit
GAGTGGTAGAACAGGCTCCTCTAATTCTTATTCACCGCCAAGCTTTGTAT610.1395402035914446No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAGGCACCGCCAGGTCCT610.1395402035914446No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCCGAGGCACCGCCAAGTCCT600.13725265927027336No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTACCC580.13267757062793092No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCAT580.13267757062793092No Hit
GAGTGGTAGAACAGGCTCCTCTGGTAAGAATAAAGAACCGCCAAGTCTTT580.13267757062793092No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGAGACGGGGTATCTAATCCCAGTG570.1303900263067597No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTACCC570.1303900263067597No Hit
GAGTGGTAGAACAGGCTCCTCTCCTTACCTTATCTGCTGCTACCCAATTG550.12581493766441726No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATAAAGCACCGCCAAGTCCT540.12352739334324603No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT530.12123984902207481No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT530.12123984902207481No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCT520.11895230470090358No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCT520.11895230470090358No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGCGGGGTATCTAATCCCAGTGTACC520.11895230470090358No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGCTTCTCTGCAGATCTTTTGTGAA520.11895230470090358No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT480.10980212741621868No Hit
GAGTGGTAGAACAGGCTCCTCTGGAGGGATATGAAGCACCGCCAGGTCCT480.10980212741621868No Hit
GAGTGGTAGAACAGGCTCCTCTATACACATCTGACGCTGCCGACGAGGGG470.10751458309504747No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCGAGTCCT450.10293949445270502No Hit
GAGTGGTAGAACAGGCTCCTCTACAATTCAAGAACCTCGGGATTTCCGGA440.1006519501315338No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTACCCT440.1006519501315338No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAAGG108.437236E-4295.0295
GAACGGC108.437236E-4295.09
TGGTAAA255.2204996E-10295.04
GAGTGGT43450.0294.321081
GGTAGAA42950.0294.313175
AGTGGTA43400.0293.980442
TGGTAGA43000.0293.970954
GAACAGG42600.0293.268779
GTGGTAG43400.0291.941253
AGAACAG44250.0283.08
TAGAACA44200.0282.986427
GTAGAAC44600.0280.448436
GTGGTAA301.5570549E-9245.833313
GAAAGGC251.8616629E-7236.09
GAGTGGA202.2671842E-5221.251
ACGCGAG150.0028403646196.66666295
AACGCGA255.5202025E-5177.0295
AGTGGTG200.006715689147.52
TGCGAAA200.006715689147.5295
GAAAAGG200.006715689147.59
Produced by FastQC (version 0.11.5)