FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-162-5-1-3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-162-5-1-3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43970
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC975222.17875824425745No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG455010.347964521264498No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT35528.078235160336593No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC33737.671139413236297No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC19344.398453491016602No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC10462.3788947009324537No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG9442.1469183534227883No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA8581.9513304525813056No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC7821.778485330907437No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC7591.7261769388219241No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG7391.6806913804866954No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC7291.657948601319081No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA6311.4350693654764612No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC6291.4305208096429383No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG5661.2872413008869683No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA4260.9688423925403684No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA4250.966568114623607No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC3870.8801455537866727No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC3520.8005458267000227No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2710.616329315442347No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA2510.5708437571071184No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2500.568569479190357No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA2420.5503752558562656No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC2150.488969752103707No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACC1670.3798044120991585No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTA1640.37298157834887424No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC1580.3593359108483057No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT1540.35023879918125994No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1520.34569024334773707No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1510.34341596543097563No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA1500.34114168751421425No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG1460.3320445758471685No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTA1350.30702751876279283No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAA1330.30247896292926996No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC1240.2820104616784171No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1170.26609051626108715No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1110.25244484876051854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1050.23879918125994998No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC1050.23879918125994998No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC1040.23652490334318854No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1030.2342506254264271No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTA950.2160564020923357No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC930.21150784625881283No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT930.21150784625881283No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTA890.20241073459176712No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACC890.20241073459176712No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAA880.20013645667500568No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTA850.1933136229247214No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAAC780.1773936775073914No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC740.1682965658403457No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG720.16374801000682285No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG720.16374801000682285No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG710.1614737320900614No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC700.15919945417329998No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCACAGGGGTATCTAATCCCAGTAA670.1523766204230157No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG660.15010234250625426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAA660.15010234250625426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA650.14782806458949282No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC620.14100523083920855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT610.13873095292244714No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA610.13873095292244714No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC600.1364566750056857No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC560.12735956333864No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCTCGTGGGGTATCTAATCCCAG550.12508528542187855No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT540.12281100750511711No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC540.12281100750511711No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGT530.12053672958835569No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA530.12053672958835569No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAG520.11826245167159427No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTAA500.11371389583807141No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC500.11371389583807141No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTA490.11143961792130998No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC480.10916534000454856No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAA480.10916534000454856No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC470.10689106208778712No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGGGTATCTAATCCCAG450.10234250625426426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC450.10234250625426426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTC450.10234250625426426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTAA450.10234250625426426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAA440.10006822833750284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAGC43700.0294.324952
TTCTCAG43700.0294.324951
CTCAGCT43450.0293.98163
GCTAGAA43100.0293.288857
TCAGCTA43600.0292.970154
CAGCTAG43600.0291.955265
AGCTAGA43700.0291.28726
CTAGAAC44000.0287.289768
TAGAACA45500.0277.49459
TTCTCAT202.2672726E-5221.249981
CAACCCG150.0028404195196.66667295
CTCAGCC150.0028404195196.666673
TTCTCAC150.0028404195196.666671
CTCATCT200.0067158192147.499983
TCTCATC352.1093122E-4126.4285662
GCTAGAG403.5887008E-4110.624997
CTCAGCA601.4536314E-598.3333363
CAGCTAA455.7329214E-498.333335
AGCTAAA455.7329214E-498.333336
GCTAGAC550.001272431880.4545447
Produced by FastQC (version 0.11.5)