FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-161-6-7-4-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-161-6-7-4-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42152
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2284554.19671664452458No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT20814.936895046498386No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT20704.910799013095464No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT18694.433953311823875No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTCCTT15583.6961472765230594No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT11782.794647940785728No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT8211.9477130385272348No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTCCTT7001.6606566710950843No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT4361.0343518694249383No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT4301.0201176693869805No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT3600.854052002277472No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCCT3340.7923704687796546No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT3140.744923135319795No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACTGCCAGGTCCTT2870.6808692351489847No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTAGTT2840.6737521351300056No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT2670.633421901689125No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCTT2410.5717403681913077No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT2010.4768457012715885No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGACCG2000.47447333459859553No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG1620.3843234010248624No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGGACCGCCAGGTCCTT1510.35822736762193963No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTTAGGCACCGCCAAGTCCTT1140.27044980072119945No Hit
ATCTGTAGAACAGGCTCCTCTAGCCTTTCAACAATGTTCTGCATAGGGAT970.23011956728031885No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCAAGGTCCTT970.23011956728031885No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT960.22774720060732587No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT870.20639590055038906No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT750.17792750047447334No Hit
ATCTGTAGAACAGGCTCCTCTGTTTCCAAAGGGGCATACGGGTTAGTTTC690.16369330043651548No Hit
ATCTGTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTTCA680.1613209337635225No Hit
ATCTGTAGAACAGGCTCCTCTCCTTTCAAAGAGGATGCAGTATGGTTTCT660.15657620041753653No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT660.15657620041753653No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGCTATAAAGCACCGCCAAGTCCTT610.14471436705257165No Hit
ATCTGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT560.13285253368760674No Hit
ATCTGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT520.12336306699563485No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTC510.12099070032264188No Hit
ATCTGTAGAACAGGCTCCTCTACCGTTTCCTAAAAAAGGGCAAGGTGGGC490.1162459669766559No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT460.10912886695767698No Hit
ATCTGAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT460.10912886695767698No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT450.106756500284684No Hit
ATCTGTAGAACAGGCTCCTCTAGCCAGAATCTTGTAAAAAAATCTGCGCT440.10438413361169101No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT430.10201176693869804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG41350.0295.02
CTGTGAA206.114897E-8295.03
TCTGAGA108.436173E-4295.02
ATCTGTC108.436173E-4295.01
ATCTGTA41650.0295.01
GTGGAAC108.436173E-4295.05
GAACGGC157.1910854E-6294.999978
TCTGTAT157.1910854E-6294.999972
TCTGTAA157.1910854E-6294.999972
AACGGCT157.1910854E-6294.999979
CTGTAGA41450.0294.28833
TGTAGAA41500.0292.156624
GAACAGG41200.0291.061868
GTAGAAC41600.0290.74525
AACAGGC41350.0290.362769
TAGAACA42450.0283.186136
AGAACAG42300.0283.14427
GACAGGC251.8608989E-7236.08
AGAACGG202.2666141E-5221.257
CTGTATA202.2666141E-5221.253
Produced by FastQC (version 0.11.5)