FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-160-6-7-4-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-160-6-7-4-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42025
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2518259.9214753123141No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT37148.837596668649613No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT24525.834622248661511No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT14143.3646638905413444No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT12242.912552052349792No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT5741.3658536585365855No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT4341.0327186198691254No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT4261.0136823319452708No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCCTT2840.6757882212968471No Hit
CTAGGTAGAACAGGCTCCTCTAGCCAGAATCTTGTAAAAAAATCTGCGCT1900.45211183819155265No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT1840.4378346222486615No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG1690.40214158239143366No Hit
CTAGGTAGAACAGGCTCCTCTACTTGTGCGGGTCCCCGTCTATTCCTTTG1490.3545508625817965No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT1440.34265318262938727No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCTCCAAGTCCTT1360.3236168947055324No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT1340.3188578227245687No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGAATATGAAGCACTGCCAAATCCTC1130.26888756692444976No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT1100.26174895895300415No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT1030.2450922070196312No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTC920.21891731112433074No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT890.2117787031528852No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT870.2070196311719215No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTTT820.1951219512195122No Hit
CTAGGTAGAACAGGCTCCTCTGTCTCCTCTATTGATCCGTTGTCCCCATA750.1784651992861392No Hit
CTAGGTAGAACAGGCTCCTCTACGCACGGATCGGTGTTCCCTACTGCTAT680.1618084473527662No Hit
CTAGGTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTTCA640.1522903033908388No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATACAAAGCACTGCCAAGTCCTT610.1451516954193932No Hit
CTAGGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT500.1189767995240928No Hit
CTAGGTAGAACAGGCTCCTCTAGAGTGATATGAAGCACCGCCAGGTCCTT460.10945865556216537No Hit
CTAGGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT460.10945865556216537No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGAACCGCCAGGTCCTT450.10707911957168353No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCTTT440.10469958358120166No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT430.10232004759071982No Hit
CTAGGTAGAACAGGCTCCTCTTATGGATGACGAGAGTTATGATGCTCTGC430.10232004759071982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGC108.436067E-4295.08
AGGTTAG350.0295.03
TAGGTTA400.0295.02
CTAGGTA41400.0294.643741
TAGGTAG41250.0294.642432
GGTAGAA41250.0294.284854
AGGTAGA41350.0293.573153
AACAGGC41300.0290.357159
GAACAGG41200.0290.34598
GTAGAAC41650.0289.687875
AGAACAG42200.0284.514227
TAGAACA43000.0279.906986
CTAGGTT450.0262.222231
GGTTAGA450.0229.444464
GTTAGAA450.0229.444465
AGGTGAA202.2665572E-5221.253
TAGGTGA202.2665572E-5221.252
GAAAAGG304.6173227E-7196.666668
CTAGGAG150.002839972196.666661
AAAAGGC304.6173227E-7196.666669
Produced by FastQC (version 0.11.5)