FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-16-4-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-16-4-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12005
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT228519.033735943356938No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT143311.936693044564764No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG9507.9133694294044155No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT7566.29737609329446No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC5734.773011245314452No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG2522.099125364431487No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2492.0741357767596833No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2472.057476051645148No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2351.9575177009579343No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG1971.6409829237817575No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG1691.407746772178259No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG1261.0495626822157436No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1261.0495626822157436No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT1261.0495626822157436No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT1200.9995835068721367No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1190.9912536443148687No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1150.9579341940857976No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGTG1080.8996251561849229No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1080.8996251561849229No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1010.8413161182840483No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG1010.8413161182840483No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC1010.8413161182840483No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT980.8163265306122449No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG950.7913369429404414No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT930.7746772178259058No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT890.7413577675968347No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG620.5164514785506039No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT580.48313202832153274No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT550.4581424406497293No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTTGT540.44981257809246145No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTT500.41649312786339027No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC470.39150354019158684No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC470.39150354019158684No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTG450.3748438150770512No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC440.36651395251978347No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC440.36651395251978347No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGT430.3581840899625156No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC400.33319450229071224No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTTGT400.33319450229071224No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTT380.31653477717617656No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT360.299875052061641No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT350.2915451895043732No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT340.2832153269471054No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT330.2748854643898376No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT330.2748854643898376No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA320.26655560183256977No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCC310.25822573927530196No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG300.24989587671803418No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGGCC270.22490628904623072No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC260.21657642648896294No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA260.21657642648896294No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT250.20824656393169513No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT240.19991670137442732No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT220.18325697625989174No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTCATGTTATAAGAGACGGGGTATC220.18325697625989174No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC210.1749271137026239No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTT210.1749271137026239No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGGTATCTAATCCCAGTTGTGG190.15826738858808828No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC180.1499375260308205No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAGT180.1499375260308205No Hit
TGCTTATAGAACAGGCTCCTCTAGAGATGGGGTATCTAATCCCAGTTGTG180.1499375260308205No Hit
TGCTTATAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGTTG180.1499375260308205No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGTG180.1499375260308205No Hit
TGCTTATAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATCCC170.1416076634735527No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT170.1416076634735527No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT170.1416076634735527No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT170.1416076634735527No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG160.13327780091628488No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTTGTG160.13327780091628488No Hit
TGCTTATAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGTGG150.12494793835901709No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAA150.12494793835901709No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTTTGGGGTATCTAATCCCAGTTGT150.12494793835901709No Hit
TGCTTATAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC150.12494793835901709No Hit
TGCTTATAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTTG140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTTGTG140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGTG140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTTGTGG140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT140.11661807580174927No Hit
TGCTTATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTT130.10828821324448147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAT11950.0295.01
CTTATAG11950.0293.76573
GCTTATA11950.0293.76572
GAACAGG11900.0293.760539
TTATAGA12050.0291.32784
TATAGAA12450.0281.967865
AGAACAG12450.0280.783148
ATAGAAC12650.0275.17796
TAGAACA13050.0266.74337
CAGTTTT2350.059.00000485-89
AGAGGGA3350.059.00000420-24
AGGGATA3100.059.00000425-29
AGCAGTT2250.059.00000485-89
GCTGTGG2350.059.00000460-64
CTGTGGC2350.059.00000460-64
CCTTTGA3350.059.00000445-49
GGGATAT3100.059.00000425-29
TGTGGCT2350.059.00000460-64
CTTTGAG3350.059.00000445-49
GAGGGAT3350.059.00000420-24
Produced by FastQC (version 0.11.5)