FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-159-5-7-5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-159-5-7-5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41143
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1299731.58982086867754No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT482811.734681476800429No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG33878.232263082419852No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC14453.5121405828451984No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT13333.239919305835744No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT12453.0260311596140292No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG6951.6892302457283133No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT6761.6430498505213522No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT6681.6236054735921057No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC5781.4048562331380794No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT5571.3538147436988066No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC5181.2590234061687287No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC4781.1618015215224948No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC4621.1229127676640012No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT3820.9284689983715334No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG3670.8920107916291957No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT3580.8701358675837931No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT3490.8482609435383904No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT3080.7486085117760007No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2130.5177065357411953No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC2010.4885399703473252No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1800.43749848090805243No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC1580.3840264443526238No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT1540.37430425588800037No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC1500.364582067423377No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT1460.3548598789587536No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1460.3548598789587536No Hit
AGGAATAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCG1320.3208322193325717No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG1250.3038183895194808No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG1240.30138784240332495No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG1190.2892351068225457No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT1160.2819434654740782No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT1010.2454852587317405No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG940.22847142891864958No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT860.2090270519894028No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCGCTT850.206596504873247No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT850.206596504873247No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC840.20416595775709112No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT820.19930486352477944No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT790.1920132221763119No Hit
AGGAATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCGCT760.18472158082784434No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC760.18472158082784434No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT740.17986048659553266No Hit
AGGAATAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTCGCTTC710.17256884524706512No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG700.17013829813090928No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT670.16284665678244173No Hit
AGGAATAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTCG640.15555501543397418No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGCT630.15312446831781834No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC630.15312446831781834No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA630.15312446831781834No Hit
AGGAATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT630.15312446831781834No Hit
AGGAATAGAACAGGCTCCTCTAGGTCACGTGGGCTCGGGGTATCTAATCC610.14826337408550663No Hit
AGGAATAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCACG600.1458328269693508No Hit
AGGAATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCTT560.1361106385047274No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT550.13368009138857156No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTCGCTT550.13368009138857156No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGTC540.13124954427241572No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGCTT540.13124954427241572No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT500.12152735580779232No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCGCT490.11909680869163648No Hit
AGGAATAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCG480.11666626157548064No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT480.11666626157548064No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT470.11423571445932479No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT450.10937462022701311No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCGC450.10937462022701311No Hit
AGGAATAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTCGC430.1045135259947014No Hit
AGGAATAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC420.10208297887854556No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCGCTT420.10208297887854556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAGG40250.0290.968968
AGGAATA41100.0290.693451
GGAATAG40950.0290.677672
AACAGGC40450.0289.530279
GAATAGA41650.0285.792333
ATAGAAC42000.0279.54765
TAGAACA42200.0277.174166
AGAACAG42400.0276.214637
AATAGAA43100.0274.466344
GACAGGC150.0028397513196.666668
TGCTATA150.0028397513196.66666295
CACACGG150.0028397513196.66666295
ATCGCGA150.0028397513196.66666295
AGGAAGG255.5178214E-5177.01
GGAAGGA255.5178214E-5177.02
AAGGACT255.5178214E-5177.04
CAGCCAG200.006714242147.5295
ACGACGG200.006714242147.5295
AGGAATG453.4759832E-6131.111111
GAAGGAC403.5870215E-4110.6253
Produced by FastQC (version 0.11.5)