FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-158-6-7-4-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-158-6-7-4-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39697
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC2033251.21797616948383No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC28767.244879965740485No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC20005.038164093004509No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC17714.4612943043554925No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC15193.8264856286369247No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTAAAGCACCGCCAAGTC12943.2596921681739173No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC10662.6853414615714035No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC7771.9573267501322515No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC6721.6928231352495149No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC6321.592059853389425No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC3820.9622893417638614No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTC3450.8690833060432779No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC3250.8187016651132327No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTC3040.7658009421366854No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC1970.49625916316094415No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTTGA1890.47610650678892613No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTC1840.4635110965564148No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTA1480.3728241428823337No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTT1160.29221351739426155No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTC950.2393127944177142No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTC790.19900748167367813No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC770.1939693175806736No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGATGCACCGCCAAGTC750.1889311534876691No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC640.1612212509761443No Hit
AATCCTATTAGAACAGGCTCCTCTACTTGTGCGGGCCCCCGTCAATTCCT490.12343502027861047No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTTGA490.12343502027861047No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC460.1158777741391037No Hit
AATCCTATTAGAACAGGCTCCTCTACTTGTGCGGGTCCCCGTCTATTCCT460.1158777741391037No Hit
AATCCTATTAGAACGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC430.10832052799959693No Hit
AATCCTATTAGAACAGGCTCCTCTAGCCAGAATCTTGTAAAAAAATCTGC420.10580144595309468No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG420.10580144595309468No Hit
AATCCTATTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC400.10076328186009018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAG108.4342854E-4295.0295
ATCCTAT39450.0295.02
CCTATTA39200.0295.04
TCCTATT39200.0295.03
TCCTATG108.4342854E-4295.03
AATCCTA39550.0294.627041
ATTAGAA39200.0293.118627
TTAGAAC39500.0290.145578
CTATTAG40050.0286.529365
TATTAGA40100.0286.172066
TAGAACA40200.0285.09339
CCAAGAA202.2656013E-5221.25295
CCTATAG150.0028393744196.666664
TCCTATA150.0028393744196.666663
GAGAGTG150.0028393744196.66666295
CTCACAA200.0067133526147.5295
CTATTAA503.2916432E-8147.55
GCAGAGA301.14079165E-4147.49998295
ATGCAGA352.1077662E-4126.428566295
ATTAAAC505.8675323E-6118.07
Produced by FastQC (version 0.11.5)