FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-157-6-7-5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-157-6-7-5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40003
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT2404860.11549133814964No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT21525.379596530260231No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT16214.052196085293603No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT15143.7847161462890284No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT7951.9873509486788492No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT6941.734869884758643No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT4701.1749118816088793No Hit
ATCTGTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTTCA3600.8999325050621204No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT3540.8849336299777516No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCTTT3460.86493512986526No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT3060.7649426293028023No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT3010.7524435667324951No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT2690.6724495662825288No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGATATAAAGCACCGCCAAGTCCTT1530.38247131465140116No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT1180.29497787665925057No Hit
ATCTGTAGAACAGGCTCCTCTCGGGAGGTTTGGCCAAAAGCGCTGGTGAA940.23498237632177585No Hit
ATCTGTAGAACAGGCTCCTCTGTTTCCAAAGGGGCATACGGGTTAGTTTC850.21248406369522285No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCCTT820.20498462615303853No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG730.18248631352648553No Hit
ATCTGTAGAACAGGCTCCTCTAGCTTAGATACGGCGGGGTTCATAGATTC700.17498687598430118No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG690.17248706347023973No Hit
ATCTGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTCT670.16748743844211683No Hit
ATCTGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT650.16248781341399396No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGATATGAAGCACCGCCAGGTCCTT620.1549883758718096No Hit
ATCTGTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT580.14498912581556384No Hit
ATCTGAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT540.13498987575931803No Hit
ATCTGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT530.13249006324525658No Hit
ATCTGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT520.12999025073119516No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACCGTCTGGATCTTGCCATTGACAA510.1274904382171337No Hit
ATCTGTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT500.12499062570307228No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC470.11749118816088792No Hit
ATCTGTAGAACGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTT470.11749118816088792No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCCTT460.11499137564682647No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCCGGTCCTT440.1099917506187036No Hit
ATCTGTAGAACAGGCTCCTCTATAGGGATATAAAGCACCGCCAAGTCCTT440.1099917506187036No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCTT440.1099917506187036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAGA108.434565E-4295.02
CCCACAA108.434565E-4295.0295
TCTGTGA108.434565E-4295.02
TCTGTAG39050.0295.02
ATCTGTA39500.0294.253171
CTGTAGA38950.0294.24263
GAACAGG38600.0291.560888
AACAGGC38800.0291.198469
TGTAGAA39050.0290.845064
GTAGAAC39100.0288.586945
AGAACAG39500.0284.917727
TAGAACA40050.0281.37336
GACAGGC351.2732926E-11252.857138
TGTAGAC403.274181E-11221.254
CTGTAAC150.0028394677196.666663
TCTGTAT150.0028394677196.666662
TCTGTAA304.6145942E-7196.666662
CGCACGA150.0028394677196.66666295
CTGTGAA150.0028394677196.666663
TAACAGG255.5167206E-5177.08
Produced by FastQC (version 0.11.5)