FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-156-6-3-8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-156-6-3-8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38753
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1830047.22215054318376No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC33638.678037829329343No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCA25646.616261966815472No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC14043.6229453203622946No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC12783.2978092018682426No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC9822.5339973679457075No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTCAATC9612.4798080148633654No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTTCGGGGTATC9212.376590199468428No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC7681.9817820555827936No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC5631.4527907516837406No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT3830.9883105824065234No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTCC2580.6657549092973447No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA2530.6528526823729776No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTCAA2480.6399504554486104No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA2280.5883415477511419No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC2110.5444739762082935No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT2030.5238304131293061No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1980.510928186204939No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGGGTATCTAATCCCAGTGTCA1850.47738239620158435No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTCAAT1660.42835393388898924No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAAT1660.42835393388898924No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTG1510.38964725311588777No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCC1400.36126235388228006No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGTCTATAAAGCACCGCCAAGTCC1290.33287745464867236No Hit
CCACGTCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC1120.2890098831058241No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAA990.2554640931024695No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTTCGGTATCTA940.24256186617810233No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTGTCAA890.22965963925373523No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC660.17030939540164633No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC640.16514850463189948No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTGTC630.16256805924702603No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGTGGGGGTATCTAATCCCAGTGT620.1599876138621526No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTTCCTAGAGGA610.15740716847727917No Hit
CCACGTCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT470.12128093308905116No Hit
CCACGTCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT470.12128093308905116No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGTGGGGTATCTAATCCCAGTGTCA460.11870048770417775No Hit
CCACGTCTAGAACAGGCTCCTCTAGACACATGCCGAGGGGTATCTAATCC450.11612004231930431No Hit
CCACGTCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT410.1057982607798106No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTTGGGGTATCT390.10063737001006373No Hit
CCACGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT390.10063737001006373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCT108.433547E-4295.02
ACGTTCT108.433547E-4295.03
CGTTCTA108.433547E-4295.04
AGACCAG108.433547E-4295.09
ACGTCGA108.433547E-4295.03
CACGTTC108.433547E-4295.02
CCACGTT206.10853E-8295.01
CCACGGC108.433547E-4295.01
GGCTAGA108.433547E-4295.05
CACGTCT38000.0293.83552
ACGTCTA38000.0293.447363
GTCTAGA37700.0293.043765
CCACGTC38500.0292.318181
CGTCTAG37850.0292.272134
CTAGAAC38000.0290.730267
TCTAGAA38000.0290.730266
AGAACAG39050.0284.04619
TAGAACA39050.0282.535228
CCACGAC251.8589708E-7236.01
GTCTAAA353.9035513E-9210.71435
Produced by FastQC (version 0.11.5)