FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-155-4-1-3-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-155-4-1-3-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38885
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG542913.961681882473961No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT481412.38009515237238No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC410510.556769962710556No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC24426.280056577086281No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC14223.656937122283657No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG12113.1143114311431144No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA10952.815995885302816No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG9462.4328147100424324No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC9102.34023402340234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC9032.3222322232223225No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC8282.1293557927221296No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG6631.705027645621705No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA5391.3861386138613863No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA4971.278127812781278No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC4471.1495435257811497No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA3520.9052333804809052No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3510.9026616947409027No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA3230.8306544940208306No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC2610.6712099781406712No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2480.6377780635206377No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTA2450.6300630063006301No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG2120.5451973768805453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAG2110.5426256911405426No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACC2060.5297672624405299No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC2010.516908833740517No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA1920.4937636620804937No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT1860.4783335476404783No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1750.45004500450045004No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTA1720.44232994728044234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG1630.41918477562041917No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1540.39603960396039606No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC1450.3728944323003729No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1430.3677510608203678No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1390.3574643178603575No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1360.3497492606403497No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAA1350.3471775749003472No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT1320.33946251768033947No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1310.3368908319403369No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1280.32917577472032916No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTA1200.3086022888003086No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGTGGGGTATCTAATCCCAGTA1150.29574386010029574No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTAA1120.28802880288028804No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACC1080.27774205992027773No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTA1060.27259868844027263No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAG930.23916677382023915No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAAC910.234023402340234No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT890.22888003086022887No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC870.22373665938022372No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG820.2108782306802109No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAG780.2005914877202006No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAA740.1903047447601903No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG730.18773305902018775No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA720.18516137328018514No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA720.18516137328018514No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGT710.1825896875401826No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC710.1825896875401826No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC700.18001800180018002No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTCGGGGTATCTAATCCCAG660.16973125884016974No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTAA650.16715957310016716No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAA650.16715957310016716No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGGGTATCTAATCCCAG640.16458788736016458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC640.16458788736016458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTAA640.16458788736016458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAA640.16458788736016458No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCACAGGGGTATCTAATCCCAGTAA620.15944451588015943No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC620.15944451588015943No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCTCGAGAGGGGTATCTAATCCC610.15687283014015688No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC600.1543011444001543No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTA560.14401440144014402No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC560.14401440144014402No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAA540.13887102996013886No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA530.13629934422013631No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCTGGGGTATCTAATCCCAGT520.1337276584801337No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTA520.1337276584801337No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC520.1337276584801337No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG500.12858428700012858No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCT500.12858428700012858No Hit
TTCTCAGCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACC500.12858428700012858No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCGGGGTATCTAATCCCAG500.12858428700012858No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCTCGTGGGGTATCTAATCCCAG480.12344091552012343No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACC470.12086922978012088No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG470.12086922978012088No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACC440.11315417256011315No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC440.11315417256011315No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCTCTGTGGGGGTATCTAATCCC410.10543911534010544No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACC410.10543911534010544No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGGTATCTAATCCCAGTAACC400.10286742960010287No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC400.10286742960010287No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGCAGCGCAGGGGTATCTAATCCC390.1002957438601003No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTAACCG390.1002957438601003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGCT108.4336306E-4295.03
GGCTAGA108.4336306E-4295.06
TTCTCAG38700.0294.618871
CTCAGCT38500.0294.233763
TCTCAGC38700.0293.85662
CAGCTAG38300.0293.459535
TCAGCTA38600.0293.089364
GCTAGAA38450.0292.31477
CTAGAAC38500.0290.785748
AGCTAGA38900.0288.933176
TAGAACA40100.0279.551159
TCGGCTA150.0028391543196.666664
GACACCG255.5155033E-5177.0295
CAGCTAA453.4735567E-6131.111115
AGCTAAA455.7278556E-498.333336
CTCAGCA550.001271311280.454553
TCAGCAG600.00179570973.749994
GCCCCTC201.8436353E-459.015-19
GTGTTCT4850.059.075-79
TTTAGGG5450.059.0115-119
Produced by FastQC (version 0.11.5)