FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-154-6-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-154-6-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36941
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2035155.09054979562005No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC21975.947321404401613No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC17384.704799545220757No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC9952.6934842045423784No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC9002.436317370942855No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC8632.336157656804093No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC6401.7324923526704745No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT4181.1315340678379038No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4111.1125849327305704No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC3650.98806204488238No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGATATGAAGCACCGCCAGGTCC3560.9636988711729515No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGAATATAAAGCACTGCCAAGTCT3000.812105790314285No Hit
CGATTCCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC2650.7173601147776184No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT2600.7038250182723802No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCCCCGCCAAGTCC1760.4764353969843805No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCC1640.4439511653718091No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA1530.41417395306028537No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATAAAGAACTGCCAGGTCC1400.3789827021466663No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC1060.2869440459110474No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG1010.27340894940580923No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTCC990.26799491080371407No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC980.2652878915026664No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT750.20302644757857125No Hit
CGATTCCTAGAACAGGCTCCTCTGTTTCCAAAGGGGCATACGGGTTAGTT680.18407731247123793No Hit
CGATTCCTAGAACAGGCTCCTCTAGCTTAGATACGGCGGGGTTCATAGAT640.17324923526704747No Hit
CGATTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT640.17324923526704747No Hit
CGATTCCTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTTT620.16783519666495222No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC580.15700711946076173No Hit
CGATTCCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT560.15159308085866652No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGTCACCGCCAAGTCC550.1488860615576189No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCC480.1299369264502856No Hit
CGATTCCTAGAACAGGCTCCTCTAAAATAGATTGTTCAGAAATATCCATC470.12722990714923796No Hit
CGATTCCTAGAACAGGCTCCTCTAGGGACCGTCTGGATCTTGCCATTGAC430.1164018299450475No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCA430.1164018299450475No Hit
CGATTCGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC410.11098779134295228No Hit
CGATTCCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT410.11098779134295228No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTTGGTCTGAGGCACCGCCAAGTCC410.11098779134295228No Hit
CGATTCCTAGAACAGGCTCCTCTCGGGCGGTTTGGCCAAAAACGCTGGTG400.10828077204190466No Hit
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC370.1001597141387618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTAG35350.0295.000034
TTTTCCT108.431915E-4295.0295
GATTCCT35700.0295.02
TTCTAGA157.183824E-6295.04
ATTCATA108.431915E-4295.03
CGATTCG108.431915E-4295.01
CGATTCC36050.0294.590851
ATTCCTA35600.0294.58573
TCCTAGA35400.0294.583345
CCTAGAA35200.0294.161936
CTAGAAC35700.0288.389347
TAGAACA36350.0282.82678
AGAACAG36950.0278.23419
GATTCCA301.551598E-9245.833342
GATTCTA251.8577703E-7236.02
GATCGAA202.2643297E-5221.25295
AGAACGG202.2643297E-5221.259
TTCCTAA202.2643297E-5221.254
CGATTAT202.2643297E-5221.251
TCCTAAT150.002838578196.666675
Produced by FastQC (version 0.11.5)