FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-153-6-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-153-6-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36470
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC2041755.98299972580203No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC400410.978886756238005No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC17694.850562105840417No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC13423.679736769947902No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC8162.237455442829723No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC7482.0510008225939127No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC4111.126953660542912No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC3951.0830819851933096No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC2700.7403345215245407No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2440.6690430490814369No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGCCTGAGGCACCGCCAAGTC2300.6306553331505347No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTTCCTAAAGGATTCAAATAGGTT2110.5785577186728819No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAGAGCACCGCCAAGTC1880.5154921853578284No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC1320.3619413216342199No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTA1230.3372635042500685No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCT1130.30984370715656706No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC970.2659720318069646No Hit
AATCCTATTAGAACAGGCTCCTCTCACCGCAATTGGACATCCCTTGCCGT870.2385522347134631No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCGAGTC760.20839045791061148No Hit
AATCCTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC650.1782286811077598No Hit
AATCCTATTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC640.17548670139840963No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCT590.1617768028516589No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTC550.1508088840142583No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT530.14532492459555799No Hit
AATCCTATTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC530.14532492459555799No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC520.14258294488620785No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTC420.11516314779270634No Hit
AATCCTATTAGAACAGGCTCCTCTAGAAGGATGTAAAGCACCATGAATTC400.10967918837400603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA206.103619E-8295.08
TTAGAAA108.431493E-4295.08
ATCCTAA206.103619E-8295.02
CCAATTA108.431493E-4295.04
CCTATTG108.431493E-4295.04
CCTATTA35050.0295.04
ATCCATT108.431493E-4295.02
CTTTAGA157.1831055E-6295.05
TCCTAAT206.103619E-8295.03
ATTAGAC206.103619E-8295.07
CAATTAG108.431493E-4295.05
CCATTAG108.431493E-4295.04
TCCAATT108.431493E-4295.03
ATCCTAT35400.0294.58332
TCCTATT35300.0294.582153
AATCCTA35850.0294.177121
ATTAGAA34750.0293.726627
TTAGAAC35050.0289.950078
CTATTAG35700.0286.73675
TATTAGA35750.0286.335666
Produced by FastQC (version 0.11.5)