FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-152-6-7-5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-152-6-7-5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36089
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC2229861.78613982099809No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC23686.561556152844357No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC12643.5024522707750285No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC9652.673944969381252No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC7512.080966499487378No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC7322.0283188783285766No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC6691.8537504502757072No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC6041.6736401673640167No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC5881.6293053284934467No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3831.0612652054642688No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC2450.6788772202056028No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGTCTGAGGCACCGCCAAGTC1980.5486436310233035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTC1280.35467871096455983No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTAGGGCACCGCCAAGTC1270.35190778353514923No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTC670.1856521377705118No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC670.1856521377705118No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCGGGATATGAAGCACCGCCAGGTC570.15794286347640554No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC560.15517193604699492No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCT560.15517193604699492No Hit
TTCTCAGCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC520.14408822632935242No Hit
TTCTCAGCTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC510.1413172988999418No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG490.13577544404112057No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTC480.13300451661170995No Hit
TTCTCAGCTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC460.1274626617528887No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGGAGCACCGCCAGGTC450.12469173432347806No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGTGGGTGGTGCCTTGTGTGTTCC440.12192080689406744No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGC390.10806616974701432No Hit
TTCTCGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC390.10806616974701432No Hit
TTCTCAGCAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC380.1052952423176037No Hit
TTCTCAGCTAGAACAGGCTCCTCTGCTTGTGCGGGTCCCCGTCTATTCCT370.10252431488819308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT35450.0295.000033
CAGCTAG35200.0295.05
TCAGCTA35400.0295.04
TTCTCAT157.1824506E-6295.01
CTCAGCG108.4311096E-4295.03
CTAGACC108.4311096E-4295.08
TCTCAGC35700.0295.02
TTCTCAG35750.0294.999971
GCTAGAA35000.0294.157177
AGCTAGA35350.0293.748236
CTAGAAC35050.0292.895848
TAGAACA35850.0285.94849
CACGTAA150.0028383075196.66667295
TCTCATC150.0028383075196.666672
ACGGAGG150.0028383075196.66667295
GCTAGAC553.0559022E-10160.909097
CAACCGA200.006710837147.5295
AGAACCA200.006710837147.5295
TCAGCGA200.006710837147.54
AGTCAGA200.006710837147.5295
Produced by FastQC (version 0.11.5)