FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-151-5-1-3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-151-5-1-3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35418
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1058629.88875712914337No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT383010.813710542661923No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTAA20515.7908408154045965No Hit
AGGAATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCTG16354.616296798238184No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT11893.3570500875261167No Hit
AGGAATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGAT11673.2949347789259695No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTAA9182.5919024224970353No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT7662.1627421085323846No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT5321.5020610988762777No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATCT4711.3298322886667795No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGAT4591.2959512112485176No Hit
AGGAATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCGA4171.177367440284601No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT4011.1321926703935852No Hit
AGGAATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGATC3971.1208989779208312No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC3661.033372861256988No Hit
AGGAATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT3500.9881980913659721No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT3390.9571404370658987No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCGA3040.8583206279293015No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCGA2600.7340900107290079No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT2560.7227963182562538No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTAA2550.7199728951380654No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAAC2190.6183296628832797No Hit
AGGAATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTAA1780.5025693150375515No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAACC1650.4658648145011012No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTA1600.45174769891015865No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTA1570.4432774295555932No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1500.42351346772827375No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA1480.4178666214918968No Hit
AGGAATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGAT1450.40939635213733133No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTA1440.40657292901914277No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTAA1420.40092608278276587No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT1320.3726918516008809No Hit
AGGAATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAAC1090.30775311988254556No Hit
AGGAATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCGA1030.2908125811734147No Hit
AGGAATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAAC980.2766954655824722No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT920.25975492687334123No Hit
AGGAATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGAT880.24846123440058726No Hit
AGGAATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG870.24563781128239878No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT830.2343441188096448No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT750.21175673386413688No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTAACCG750.21175673386413688No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTAAC730.2061098876277599No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA730.2061098876277599No Hit
AGGAATAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTA690.19481619515500592No Hit
AGGAATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAACCG660.18634592580044046No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG640.18069907956406345No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT610.172228810209498No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTAA590.166581963973121No Hit
AGGAATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCAG590.166581963973121No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT580.16375854085493252No Hit
AGGAATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAACCG540.15246484838217855No Hit
AGGAATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAACC540.15246484838217855No Hit
AGGAATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCGAT530.14964142526399007No Hit
AGGAATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAGT520.14681800214580157No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGATC470.13270088655485912No Hit
AGGAATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAAC470.13270088655485912No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT460.12987746343667061No Hit
AGGAATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT440.12423061720029363No Hit
AGGAATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACCG430.12140719408210514No Hit
AGGAATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTAAC430.12140719408210514No Hit
AGGAATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTA420.11858377096391666No Hit
AGGAATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA400.11293692472753966No Hit
AGGAATAGAACAGGCTCCTCTAGCTCAGATGGTATGAGAGACGGGGTATC400.11293692472753966No Hit
AGGAATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT390.11011350160935118No Hit
AGGAATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACCG390.11011350160935118No Hit
AGGAAGGACTACCGGGGTATCTAATCCCAGTAACCGATCTGTCTCTTATA370.1044666553729742No Hit
AGGAATAGAACAGGCTCCTCTAGACGTCAGGGGTATCTAATCCCAGTAAC370.1044666553729742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAGG108.4304676E-4295.08
AACGGCT108.4304676E-4295.09
GAACGGC108.4304676E-4295.08
GGAATAG35100.0292.898862
AGGAATA35300.0292.07511
GAACAGG34800.0291.60928
AACAGGC34750.0291.179849
GAATAGA35850.0286.359833
ATAGAAC35800.0285.111765
TAGAACA35800.0283.87576
AATAGAA36650.0279.304234
AGAACAG36800.0276.161687
TAGAGGA150.002838092196.66666295
ACCAGGC150.002838092196.666669
CAAGAGG150.002838092196.66666295
AGGAAGG359.929936E-7168.571431
GGAAGGA359.929936E-7168.571432
CAGAAGG352.1058705E-4126.428566295
AAGGACT505.8587448E-6118.04
GAAGGAC559.409159E-6107.272723
Produced by FastQC (version 0.11.5)