FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-150-6-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-150-6-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34099
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC2245765.8582363119153No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC17004.985483445262324No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC15754.618903780169506No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC15084.4224170796797555No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4821.413531188597906No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC4171.2229097627496406No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC3991.170122290976275No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC2400.7038329569782106No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCC1930.565999002903311No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCC1280.3753775770550456No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATGTGAAGCACCGCCAAGTCC1150.33725329188539255No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCC960.2815331827912842No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAGCAGCATCAAATCCT930.2727352708290566No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT890.2610047215460864No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGGTATAAAGTACCGCCAAGTCC840.2463415349423737No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT690.20235197513123554No Hit
GCGAAGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT670.19648670048975042No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG610.17889087656529518No Hit
GCGAAGTTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT600.17595823924455264No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA550.1612950526408399No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC550.1612950526408399No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC500.1466318660371272No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT500.1466318660371272No Hit
GCGAAGTTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT490.14369922871638466No Hit
GCGAAGTTGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT450.13196867943341448No Hit
GCGAAGTTAGAACAGGCTCCTCTATAAATATAGGGTTTGAGCAGGACTTT430.12610340479192939No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGATATAAAGCACTGCCAAGTCC400.11730549282970176No Hit
GCGAAGTTAGAAAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT390.1143728555089592No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC350.10264230622598902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTTA33150.0295.000033
CATCCTG108.429054E-4295.0295
GAAGTTG108.429054E-4295.03
GCGAAGC108.429054E-4295.01
GCGAAAT108.429054E-4295.01
AAGGTAG108.429054E-4295.04
GCGAAGG157.178949E-6294.999971
GCGAAGT33550.0294.560331
CGAAGTT33400.0294.55842
AAGTTAG32950.0294.552344
AGTTAGA33000.0294.106055
GTTAGAA32800.0294.10066
TTAGAAC32750.0292.748087
AGAACAG33900.0283.687329
TAGAACA34150.0282.042458
AAGTTAA301.54796E-9245.833314
GTTAAAC251.8556602E-7236.06
GTTAGAC202.2627954E-5221.256
TTAGAAG150.0028376179196.666667
AGGTAGA150.0028376179196.666665
Produced by FastQC (version 0.11.5)