FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-15-4-4-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-15-4-4-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC234919.938884644766997No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT140311.909006026653085No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTACCCTG9007.639419404125286No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTACC5744.87225193107546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC5504.6685340802987865No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTAC2892.4531024531024532No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT2722.3088023088023086No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTACCC2061.7485782191664543No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA1941.6467192937781174No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTACCCT1931.6382310499957558No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC1771.502419149477973No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC1761.4939309056956116No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTGTA1621.3750954927425516No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTACC1411.1968423733129614No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTG1401.1883541295306002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1381.1713776419658772No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTAC1241.0525422290128172No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1090.9252185722773958No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTAC1060.8997538409303115No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1050.8912655971479502No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTG950.8063831593243358No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTACC910.7724301841948901No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC690.5856888209829386No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG680.5772005772005772No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC680.5772005772005772No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT680.5772005772005772No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC670.5687123334182157No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGT650.5517358458534929No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGT600.5092946269416858No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTA600.5092946269416858No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA580.49231813937696295No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTA580.49231813937696295No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTG570.48382989559460143No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGTCAGGGGTATCTAATCCCAGT540.4583651642475172No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTACC520.44138867668279436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTG510.4329004329004329No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTACC450.38197097020626436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGT450.38197097020626436No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTACC370.3140650199473729No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCCA360.30557677616501144No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC360.30557677616501144No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTAC340.2886002886002886No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG330.2801120448179272No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC320.27162380103556577No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC300.2546473134708429No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATCC290.24615906968848147No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTGT270.2291825821237586No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT270.2291825821237586No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG270.2291825821237586No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTCGGGGGTATCTAATCCCAGTGT260.22069433834139718No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACGGGGGTATCTAATCCCAGTGTA250.21220609455903572No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTGTA230.19522960699431288No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTG220.18674136321195145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT210.17825311942959002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTACC210.17825311942959002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC210.17825311942959002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTGACATTGGGGTATCTAATCCCA200.16976487564722859No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGTAGGGGTATCTAATCCCAGTGTA190.16127663186486715No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTG180.15278838808250572No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTTGGGGTATCTAATCCCAGTGTAC180.15278838808250572No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATC180.15278838808250572No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTG170.1443001443001443No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCTGGGGTATCTAATCCCAGTGTA170.1443001443001443No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTA170.1443001443001443No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATTGTCGGGGTATCTAATCCCAGTG170.1443001443001443No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTGTAC160.13581190051778289No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGGTATCTAATCCCAGTGT160.13581190051778289No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTG160.13581190051778289No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTTGGGGTATCTAAT160.13581190051778289No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTGTA150.12732365673542145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT150.12732365673542145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG150.12732365673542145No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA140.11883541295306002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC120.10185892538833714No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGCTGTTATAAAATCGCCATCATAT120.10185892538833714No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGCGTCTGGGGTATCTAATCCCAG120.10185892538833714No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTATCTAATCCCAGTGTACCCTGTC120.10185892538833714No Hit
GCGAAGTTAGAACAGGCTCCTCTGACGGGGGTATCTAATCCCAGTGTACC120.10185892538833714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT11700.0295.01
GAAGTTA11700.0293.739323
CGAAGTT11750.0292.489352
GTTAGAA11700.0292.478646
AAGTTAG11700.0292.478644
AGTTAGA11700.0292.478645
TTAGAAC11550.0292.44597
TAGAACA12100.0279.15298
AGAACAG12200.0275.655739
TTAGAAA202.2252914E-5221.257
AGAAAGG150.0028140582196.666679
GGTACGA150.0028140582196.66667295
ACGACCA200.0066536483147.5295
CACCGCC3450.059.00000835-39
ACCGCCA3450.059.00000835-39
GCCCTCG257.742468E-659.00000425-29
TCCTTTG3600.059.00000445-49
AGGGGGT150.00435987759.00000420-24
TGTTGCT501.8189894E-1259.00000465-69
TTGTTGC150.00435987759.00000460-64
Produced by FastQC (version 0.11.5)