FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-149-4-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-149-4-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34971
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT526415.052472048268564No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC445512.73912670498413No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG31949.133281862114323No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT20035.727602870950216No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT19985.7133053101140945No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC14304.0891023991307085No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT10442.9853307025821394No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT8532.4391638786423036No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC5651.6156243744817136No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT5561.5898887649766948No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG5291.5126819364616395No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT4721.349689742929856No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4661.3325326699265105No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC4391.2553258414114552No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT4141.1838380372308484No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC3440.9836721855251495No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTT3360.9607960881873552No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2820.8063824311572445No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC2760.7892253581538989No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTC2700.7720682851505534No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTT2420.6920019444682737No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG2010.5747619456120786No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2010.5747619456120786No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1890.5404477996053874No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCT1870.5347287752709388No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTT1810.5175717022675931No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC1790.5118526779331446No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1790.5118526779331446No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC1670.47753853192645335No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC1660.47467901975922905No Hit
CCACGTCTAGAACAGGCTCCTCTAGTATCCATCGTTTACGGCTAGGACTA1560.44608389808698634No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAG1540.4403648737525378No Hit
CCACGTCTAGAACAGGCTCCTCTAGGAGGGGTATCTAATCCCAGTCGCTT1510.43178633725086496No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1500.4289268250836407No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCCGTGGGGGTATCTAATCCCAG1500.4289268250836407No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTT1450.4146292642475194No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1340.3831746304080524No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA1340.3831746304080524No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1290.36887706957193106No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGCGGGGTATCTAATCCCAGTCGCT1270.36315804523748246No Hit
CCACGTCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTT1240.3545795087358097No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1210.34600097223413684No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTCGCT1190.3402819478996883No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC1160.3317034113980155No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC1090.3116868262274456No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA1030.29452975322409997No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTCG1000.2859512167224272No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCAGCAGGGGTATCTAATCCCAGTC920.26307511938463296No Hit
CCACGTCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT920.26307511938463296No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGGGGGTATCTAATCCCAGTCG880.25163707071573593No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT850.2430585342140631No Hit
CCACGTCTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTCGCTT840.2401990220468388No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC820.23447999771239025No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC810.231620485545166No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGC810.231620485545166No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTC770.2201824368762689No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC750.21446341254182036No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGGTATCTAATCCCAGT750.21446341254182036No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCG720.20588487604014755No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTCG720.20588487604014755No Hit
CCACGTCTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTC670.1915873152040262No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGT650.18586829086957765No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC630.1801492665351291No Hit
CCACGTCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT620.17728975436790484No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTCGGGGTATCTAATCCCAGTCGC610.17443024220068057No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC590.168711217866232No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT540.15441365703011067No Hit
CCACGTCTAGAACAGGCTCCTCTAGCACATGGGGTATCTAATCCCAGTCG540.15441365703011067No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTCGCT530.1515541448628864No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTC530.1515541448628864No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTA510.14583512052843786No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGCTCGGGGTATCTAATCC510.14583512052843786No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTAGTGGGCTTGGGGTATCTAAT490.1401160961939893No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT490.1401160961939893No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA470.13439707185954078No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT370.10580195018729806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTC34550.0294.146151
CGTCTAG34250.0293.277374
GTCTAGA34200.0293.274845
CACGTCT34500.0292.86232
ACGTCTA34450.0292.859223
TCTAGAA34350.0291.56486
CTAGAAC34500.0290.29717
TAGAACA36250.0276.689648
AGAACAG36700.0273.2979
CCACGTT200.0067099663147.499981
CCACGCT301.1396404E-4147.499981
CCACAGG200.0067099663147.49998295
ACGCTAG455.722986E-498.333333
CACGTCA508.69927E-488.52
CGCTAGA650.002464606268.076934
CGCAGAG700.003306107463.21429295
TATTTTT452.7284841E-1159.00000495-99
TTCTATC1300.059.000004100-104
AGCGTTT452.7284841E-1159.00000460-64
GTATTTT452.7284841E-1159.00000495-99
Produced by FastQC (version 0.11.5)