FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-148-5-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-148-5-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34125
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1164134.11282051282051No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT356910.458608058608059No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCG22336.543589743589743No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTGTC12143.5575091575091573No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT10553.0915750915750917No Hit
CTAGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTTCT9342.736996336996337No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT7842.2974358974358977No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT5551.6263736263736264No Hit
CTAGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTCTG4011.1750915750915751No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTTCT3951.1575091575091576No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCG3911.1457875457875457No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT3591.052014652014652No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCTGT3491.0227106227106229No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT3390.9934065934065934No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCTTC3220.9435897435897437No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC2980.8732600732600732No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC2550.7472527472527473No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT2540.7443223443223443No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCTTC2440.715018315018315No Hit
CTAGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2330.6827838827838828No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCTTC2030.5948717948717949No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC1810.5304029304029304No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCGCT1650.4835164835164835No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG1610.47179487179487184No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT1470.43076923076923074No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCG1450.4249084249084249No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCG1390.40732600732600727No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT1380.4043956043956044No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1280.3750915750915751No Hit
CTAGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTTCT1210.3545787545787546No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCGC1150.336996336996337No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTTCT1130.3311355311355312No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTCG1080.3164835164835165No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCGCT1030.30183150183150187No Hit
CTAGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGCTT1030.30183150183150187No Hit
CTAGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG1020.2989010989010989No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGGTATCTAATCC940.2754578754578755No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGCTT860.252014652014652No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT860.252014652014652No Hit
CTAGGTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTCGC850.2490842490842491No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT800.23443223443223443No Hit
CTAGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTTCT780.2285714285714286No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG780.2285714285714286No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC770.22564102564102562No Hit
CTAGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCGCTTC710.20805860805860804No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCGC700.20512820512820512No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTCG700.20512820512820512No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT690.2021978021978022No Hit
CTAGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCTTC690.2021978021978022No Hit
CTAGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTTCT680.19926739926739928No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT660.19340659340659339No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCGC620.18168498168498168No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT610.17875457875457876No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCGCTTCT610.17875457875457876No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT600.1758241758241758No Hit
CTAGGTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCAGT570.16703296703296705No Hit
CTAGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCTT550.16117216117216118No Hit
CTAGGTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCTTC540.15824175824175826No Hit
CTAGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGGG530.15531135531135531No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA530.15531135531135531No Hit
CTAGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGTC510.14945054945054945No Hit
CTAGGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT490.1435897435897436No Hit
CTAGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGCTT490.1435897435897436No Hit
CTAGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTTCT470.13772893772893774No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAATC470.13772893772893774No Hit
CTAGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTCGCTTCTG450.13186813186813187No Hit
CTAGGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCGCT440.12893772893772895No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACTGCCAAGTCCTT430.126007326007326No Hit
CTAGGTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTCGC420.12307692307692308No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTT410.12014652014652014No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGTGGGGTATCTAATCCCAGTCG410.12014652014652014No Hit
CTAGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTCGCT380.11135531135531135No Hit
CTAGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATTGGGGTATCTAAT380.11135531135531135No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCGC360.10549450549450548No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCTT360.10549450549450548No Hit
CTAGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGCTT350.10256410256410256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTA157.17904E-6295.02
AGGTTAG157.17904E-6295.03
CTAGGAA108.429109E-4295.01
GAGGCAA108.429109E-4295.0295
GGTAGAA33400.0294.558384
TAGGTAG33750.0293.251862
AGGTAGA33600.0293.244053
CTAGGTA33950.0292.82771
GAACAGG33700.0291.49858
GTAGAAC33850.0290.642555
AACAGGC33900.0289.343669
TAGAACA34750.0282.266176
AGAACAG35000.0280.671427
GGTTAGA202.2628246E-5221.254
TAGGTAA150.0028376356196.666672
AACGGCT150.0028376356196.666679
GCACAGG150.0028376356196.666678
AGGTAGC255.5096083E-5177.03
GAACGGC200.006709253147.58
GGTGAAC200.006709253147.54
Produced by FastQC (version 0.11.5)