FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-147-6-4-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-147-6-4-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34156
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC1473343.13444197212789No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC24357.129054924464223No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC20435.981379552640824No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC18895.530507085138775No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG9262.7110902916032322No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC9222.699379318421361No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC7702.2543623375102473No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTC6391.8708279658039582No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTCTGAAGCACCGCCAAGTC5921.7332240309169693No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC4071.1915915212554162No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTC3671.0744817894367023No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATAAAGAACTGCCAGGTC3210.939805597845181No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT2800.8197681227309989No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGAGACGGGGTATCTAATCCCAGT2580.7553577702307062No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT2380.6968029043213491No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCGAGTC2230.6528867548893313No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG2120.6206815786391849No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA2090.6118983487527814No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTTGTGG2020.5914041456845064No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT2010.5884764023890385No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTT1930.5650544560252957No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC1910.55919896943436No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG1880.5504157395479564No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCC1850.5416325096615529No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1690.49478861693406717No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCATGTC1570.459655697388453No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1550.45380021079751726No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTG1460.4274505211383066No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG1300.38060662841082094No Hit
TCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC1230.36011242534254595No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCATGGGGTATCTAATCCCAGTTGT1210.3542569387516103No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG1180.3454737088652067No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1120.3279072490923996No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1090.31912401920599603No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTG1090.31912401920599603No Hit
TCTCAATCTAGAACAGGCTCCTCTATAGGGATATGAAGCACCGCCAGGTC1070.3132685326150603No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC1050.3074130460241246No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT1000.29277432954678534No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1000.29277432954678534No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG810.23714720693289612No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGAGACTGTCTCTTATAGGGGT620.1815200843190069No Hit
TCTCAATCTAGAACAGGCTCCTCTATCTGCCTCTGTGGGGTATCTAATCC590.17273685443260334No Hit
TCTCATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC500.14638716477339267No Hit
TCTCAATCTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC380.11125424522777842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGCT350.0295.000032
CGCGAAA108.429163E-4295.0295
CTAGACC108.429163E-4295.08
CTCAATC33450.0295.02
TCAATCT33450.0294.559053
AATCTAG33100.0294.108765
CAATCTA33350.0293.67324
ATCTAGA33200.0293.222876
CTAGAAC33100.0290.543828
TCTCAAT34050.0290.234951
TCTAGAA33200.0289.22447
TAGAACA34300.0279.088939
TCTCAGC400.0258.1251
CAGCTAG351.2732926E-11252.857164
TCTAGAC500.0236.07
TCAGCTA450.0229.444463
CTCATCT202.2628536E-5221.252
CAATCTG255.509679E-5177.04
GCTAGAA553.0559022E-10160.909096
TAGAACG200.0067092963147.59
Produced by FastQC (version 0.11.5)